Orflo MOXI GO, Руководство пользователя, Страница: 18 / 50

Moxi GO™ User Guide
Page 14
5. Select “Start” or insert a new cassette and close the door to begin sample
acquisition. See “General Instructions for Running a Test” section for further detail.
Running a “Cell Cycle” Test
The Cell Cycle assay can be used to assess the proliferation states of a monodisperse
population of cultured mammalian cells, categorizing cells into three general phases of mitosis:
G0/G1, S, and G2/M phases. It is important to note that the cell cycle assay on Moxi GO™ has
been configured to run specifically with the Orflo Technologies, LLC Cell Cycle Protocol, thereby
insuring that the sample fluorescence intensity is appropriately matched to the fluorescent gain
settings of the assay/instrument. Beyond that, it is also critical, with all cell cycle preps, to
prepare the cells into a single cell suspension. Achieving this may require protease treatments
and mechanical trituration to break apart clusters. It may also be necessary to filter the sample
to remove more stubbornly aggregated cell clusters.
To run a Cell Cycle assay, touch the associated icon from the Home screen (image below/left).