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Moxi GO™ User Guide
Page 9
Fluorescence Gain
The Fluorescence Gain field can be set to adjust the level of fluorescence gain for the “Open
Flow Cytometry” assay only. Four possible values ( Low, Default, Medium, High ) can be entered
here and will seed the value of the dropdown list on the “Open Flow Cytometry” application start
screen. The need to change this setting is uncommon as the
Default setting is designed to
properly display the majority of sample preparations. However, for extremely bright samples, it
may be necessary to lower the fluorescence gain to the
Low setting to prevent the PMT from
saturating. When a sample is too bright, the user will see points accumulating at the top of the
scatter/dot plot or, in cases of complete saturation, all the point accumulating in a line at the 0
mV level on the scatter plot. To set the fluorescence gain, select the
Settings icon and then
use the left and right arrows on the screen to highlight the Fluorescence Gain field. Use up or
down arrows to change and select the field value, and then select Done.
Note: the user can also make a one-time (per-test) change to the fluorescent gain value
of the “Open Flow Cytometry” by touching the value on the test start screen as described
in Step 3 of the “Analyzing a Sample” | “Running a Test” section.
Auto Noise-Find
This field sets the behavior of the system with respect to identifying system noise during tests.
There are two possible settings:
Yes or No . If set to Yes , the system applies an algorithm to
each test, after acquisition, to try to identify the noise (and small-particle “debris”) in the sample.
If set to
No , the system will use a hard-coded values for the noise location for each test, capping
the noise area to a region defined by events <2.9 µ m AND fluorescence levels <10mV.
To set the Auto Noise-find, select the Settings icon and then use the left and right arrows on
the screen to highlight the Auto Noise-find field. Use up or down arrows to change and select
the field value to Off or On, and then select Done.