Orflo MOXI GO, Руководство пользователя, Страница: 20 / 50

Moxi GO™ User Guide
Page 16
at the rightmost (brightest, or highest fluorescence MFI) peak(s) cluster on the histogram . After
gating the cell population, hitting the “Next” button brings the user to linear-scaled view (image
below/middle) of that gated region or region of interest (ROI). This is the view that will be used
to define the cell cycle phases. Prior to gating those phases, the user can further refine the ROI
by either using the arrows immediately below the x-axis to change the upper and/or lower scale
ranges or by hitting Back to go back to the Logicle scale view for more-coarse adjustments.
When satisfied with the scaling, the user can then touch-and-drag the green markers to enclose
the left-most peak that is the G0/G1 region. Touching Next then enables the blue gate markers
(image below/right), allowing for the demarcation of the G2/M region. Note, in gating these
regions, it is often helpful to refer to the “ratio” column in the table below (red brackets in image
below/middle and below/right). Gates should be adjusted such that the G2/M region is ~2x the
fluorescence ratio of the G0/G1 region. The calculated cell counts, percentages, MFIs, and MFI
ratios (to the G0/G1 region MFI) for each cell cycle region are presented in the table below the
histogram. Hitting Save will then save the control file and auto-prompt the user to insert a new
cassette to run a Treated sample. If no additional samples need to be run, the user can simple
touch Cancel to exit back to the Home screen.
Treated Sample
After running a new control sample, the user is automatically prompted (image below/left/top) to
load a new cassette to run a treated sample. Treated samples can also be run asynchronously
by selecting a prior run test (image below/left/bottom) to use as a control. Either way, upon
completion of the treated sample acquisition, a linear-scale view of the PI fluorescence
histogram is presented (image below/right). The extents of this region (x-axis scale) are
predefined by the control sample to which this sample is being compared. Likewise, the G0/G1
and G2/M gating are predetermined by the control file reference. All relevant information for
those regions is presented in table below the histogram, including: the calculated cell counts,
percentages, MFI’s, and MFI ratio’s (to the G0/G1 region MFI).