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OM0249
Page 15 of 64
Rev 4 June/2020
7.6.3
CO
2
/ N
2
Supply
•
CO
2
/
N
2
of medical grade is recommended.
•
A two-stage pressure regulator, Linde # 19590, or equal, is recommended.
•
DO NOT USE a single stage regulator.
It will not give a stable output at 20 psi and exposes the Incubator to the gas cylinder pressure.
•
High concentrations of CO
2
gas can cause asphyxiation!
•
Install Incubator in a well-ventilated area.
•
Gas Supply pressure to the Invitrocell is rated at 20psi (1.4 bar).
•
Do not exceed 25psi (1.8 bar) or damage to the incubator may occur.
This Incubator is designed to be operated with CO
2
gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO
2
/
N
2
should not be connected to this equipment.
CO
2
gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve.
Check the gas cylinder for the proper identification labels.
Do not use CO
2
gas cylinders equipped with siphon tubes.
A siphon tube is used to extract liquid CO
2
from the cylinder which can damage the pressure regulator.
Consult with your gas supplier to ensure that the CO
2
cylinder does not contain a siphon tube.
7.6.4
CO
2
/ N
2
Pressure Regulators
The regulator’s high-pressure stage direct from the supply cylinder must have a range of from 0 to 2000 PSI or
0 to 140 BAR. This gauge indicates actual tank pressure.
The low-pressure stage should have a range of 0 to 30 PSI or 0 to 2 BAR (100 PSI or 6 BARS maximum). This gauge
will indicate the actual CO
2
/N
2
pressure to the Incubator.
Some single stage CO
2
/N
2
pressure regulators have two gauges. USE A TWO-STAGE REGULATOR.
All NuAire Incubators use CO
2
/N
2
in such quantities that precise metering of CO
2
/N
2
input pressure is important for
maximum performance.
To connect the regulator:
First: Open the CO
2
cylinder slightly, for an instant (this is termed “cracking the valve.”) This will blow out dust
or dirt that may have collected in the valve outlet.
BE SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt.
Second: Make sure the regulator pressure-adjusting screw is released by turning it counterclockwise until it turns
freely.
Third: Attach the regulator to the cylinder valve and tighten the connection nut with a wrench.
BE SURE DISC SEAL IS IN PLACE BEFORE MAKING CONNECTION.
7.6.5 CO
2
/ N
2
Supply Adjustment
With the regulator OFF (i.e. fully counterclockwise), open the cylinder valve slowly usually 1 to 2 turns is sufficient.
NEVER STAND IN FRONT OR BEHIND THE REGULATOR WHEN
OPENING THE VALVE. ALWAYS STAND TO ONE SIDE.
The cylinder tank pressure should read: 700 to 800 PSI (48 to 55 BAR) for CO
2
or 2550 to 2650 PSI (176 to 183 BAR)
for N
2
more or less depending on the temperature of the cylinder.
Next turn each regulator's pressure adjusting knob clockwise until the low-pressure gauge reads 20 PSI or 1.4 BAR.
Both gas connections are now complete.
Note
:
OSHA requires the CO
2
tanks to be physically restrained (i.e. via chained to wall)
to prevent accidental damage to cylinder.
7.6.6 Checking the Connections
The connections can be checked for leakage by brushing a small amount of soapy water on each one.
Observe to see if any bubbles are blown. If not the connection is secured properly.
If it is, the cause of the leaking needs to be determined, (i.e. loose clamp or damaged hose) and corrected.
CAUTION
!
CAUTION
!
Содержание NU-5710
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