Chapter 3 Usage of Components
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3.4.4 Using an Oil Immersion Condenser Lens
When using the TI2-C-LHO HNA oil lens, use
immersion oil to immerse between the prepared
specimen slide and the top lens.
Spread immersion oil, about the size of the
diameter of the tip of the condenser lens, on the
glass slides (or cover glass) of the prepared
specimen, and then slowly lower the condenser
lens so that the tip of the lens touches the oil.
Oil immersing of the condenser
Notes on using an oil immersion condenser lens
•
If air bubbles are mixed in the immersion oil, the quality of the microscope images will decrease. Do
not to let air bubbles get mixed in.
•
The oil thickness of 1 mm is sufficient. Do not apply too much oil.
•
When immersing the condenser in oil, use a clip to firmly hold the specimen in place.
Otherwise, the specimen might be drawn to the condenser by the surface tension and the viscosity of
the oil.
Focusing on the oil immersion condenser
When using a high-magnification, oil immersion condenser lens, make sure to immerse the area between
the prepared specimen slide and the condenser lens before focusing on the condenser.
Using the condenser refocusing clamp
After forming the field aperture image on the specimen plane, tighten the condenser refocusing clamp, and
mark the power limit of the condenser. This prevents the tip of the condenser lens from colliding with the
specimen and affixes the field aperture image.
Wiping off oil
Oil remaining at the tip of the oil-immersion lens after the wiping or oil adhering to the tip of the dry lens will
adversely affect the image. After use, thoroughly wipe off all oil, and make sure that no oil adheres to the
tips of other objectives.
•
When wiping off oil, use a lens tissue or clean cloth moistened with benzine and gently wipe off a few
times. To wipe off oil cleanly, be sure to use the same portion of the tissue or cloth only once. For a
clean finish, use absolute alcohol (ethyl alcohol or methyl alcohol) at the end.
•
If petroleum benzine is unavailable, use methyl alcohol instead. However, because the cleaning power
of methyl alcohol is weak, the user may need to wipe several times (usually three or four times).
•
When wiping oil off the specimen, take due care not to damage the specimen.
CAUTION
Take due care when handling absolute alcohol or petroleum benzine, as they are highly inflammable.
Do not bring any ignition source or use the power switch near absolute alcohol or petroleum
benzine.
Immersion oil
HNA oil lens
