Chapter 2 Microscopy
18
2.2 Diascopic Phase Contrast (Ph) Microscopy
1
Focus on the sample with BF
microscopy.
1.1 Perform the procedure from step
1
to step
3
in “2.1 Diascopic Bright-field (BF) Mi-
croscopy.”
Configuration of the microscope
In addition to the configuration for BF microscopy, a Ph
objective and a condenser module that have the same Ph
code are used.
Place a phase ring of the objective and an annular
diaphragm of the condenser module into the optical path.
This causes diffraction and interference of light which gives
contrast to the colorless and transparent image of the
sample, enabling microscopy observation.
Note: Letters within [ ] correspond to those in the procedure.
2
Set up for Ph microscopy and adjust the phase
plate ring and annular diaphragm.
2-1 Change the objective to the objective for Ph
microscopy.
[a]
Nosepiece
(
☞
3.9.1)
The phase plate ring enters the optical path.
Memorize the Ph code of the objective.
▼
2-2 When using a liquid immersion type
objective, perform oil or water immersion.
[b]
Objective
(
☞
3.10.2
、
3.10.3)
▼
2-3 Focus on the sample.
[c] Focus knob (
☞
3.11.1)
▼
2-4 Place the Ph condenser module into the
optical path.
[d] Condenser turret (
☞
3.4.6)
The annular diaphragm enters the optical path.
Select the condenser module having the same Ph
code as the objective.
▼
2-5 Remove one of the eyepieces, attach the
centering telescope, and focus on the annular
diaphragm image.
[e] Centering telescope (
☞
3.6.4)
▼
2-6 Overlay the annular diaphragm image on the
phase plate ring image.
[f] Annular diaphragm centering screw (
☞
3.4.7)
▼
2-7 Replace the centering telescope with the
removed eyepiece.
[e] Centering telescope (
☞
3.6.4)
[a]
[g]
[h]
[c]
[b]
[d]
[i]
[i]
[f]
[e]
