Chapter 2 Microscopy
21
2.3 Diascopic DIC / IMSI Microscopy (Continued)
2-10 Loosen the clamp screw on the polarizer.
[g]
Polarizer
(
☞
3.5.1)
▼
2-11 Turn the entire polarizer
until a dark cross (Crossed Nicols) appears.
[g]
Polarizer
(
☞
3.5.1)
▼
2-12 Tighten the clamp screw on the polarizer.
[g]
Polarizer
(
☞
3.5.1)
▼
2-13 Replace the centering telescope with the
removed eyepiece.
[e] Centering telescope in/out dial ((
☞
3.6.4)
3
Perform DIC microscopy.
3-1 Place the condenser module for DIC
microscopy or IMSI microscopy and the DIC
slider into the optical path.
[h] Condenser turret (
☞
3.4.6)
[i] DIC slider slot (
☞
3.9.2)
Use the condenser module and the DIC slider in the
proper combination. For details on combinations, see
“4.3 Details of Diascopic DIC and IMSI Microscopies.”
▼
3-2 Focus on the sample.
[c] Focus knob (
☞
3.11.1)
▼
3-3 Remove one of the eyepieces, attach the
centering telescope, and focus on the pupil of
the objective.
[e] Centering telescope (
☞
3.6.4)
▼
3-4 Adjust the size of the dia aperture diaphragm
to 70-80% of the size of the numerical aper-
ture of the objective.
[j] Aperture diaphragm open/close lever (
☞
3.4.5)
Adjust the size of the aperture diaphragm image while
observing the objective pupil plane and the aperture
diaphragm image using the centering telescope.
▼
3-5 Replace the centering telescope with the
removed eyepiece.
[e] Centering telescope (
☞
3.6.4)
▼
3-6 Adjust the size of the dia field diaphragm
image.
[k] Field diaphragm dial (
☞
3.3.3)
▼
3-7 When an objective with correction collar is
used, adjust the correction collar.
[b]
Objective
(
☞
3.10.1)
Adjust the correction collar of the objective according
to the thickness of the prepared specimen slide or the
cover glass of the culture vessel.
▼
3-8 Move the stage and turn the focus knobs to
observe the sample.
[d] Stage handle (
☞
3.8.3)
[c] Focus knob (
☞
3.11.1)
