Chapter 2 Microscopy
20
2.3 Diascopic DIC / IMSI Microscopy
1
Focus on the sample with BF
microscopy.
1-1 Check that the vessel of the sample is
suitable for DIC / IMSI microscopy.
Do not use a plastic container (
☞
4.3.1).
▼
1.2 Follow the procedure from step
1
to step
3
in “2.1 Diascopic Bright-field (BF) Mi-
croscopy.”
Configuration of the microscope
DIC microscopy is performed by the Senarmont system. A
combination of a polarizing plate (polarizer or analyzer) and
a DIC prism changes the colorless and transparent sample
into different light intensities, which enables microscopy
observation.
In addition to the configuration for BF microscopy, a polarizer,
analyzer, DIC condenser module (condenser side DIC prism),
a DIC objective, and a DIC slider (objective side DIC prism)
are used. In the case of IMSI microscopy, a condenser
module and a DIC slider, both for IMSI, are used.
Note: Letters within [ ] correspond to those in the procedure.
2
Set up for DIC microscopy / IMSI microscopy and
adjust the azimuth of the polarizing plate.
2-1 Change the objective to the objective for DIC
or IMSI microscopy.
[a]
Nosepiece
(
☞
3.9.1)
▼
2-2 When using a liquid immersion type
objective, perform oil or water immersion.
[b]
Objective
(
☞
3.10.2
、
3.10.3)
▼
2-3 Focus on the sample.
[c] Focus knob (
☞
3.11.1)
▼
2-4 Move the field of view to the portion of the
specimen where no object is visible (or the
portion of a prepared specimen slide where a
cover glass is placed).
[d] Stage handle (
☞
3.8.3)
▼
2-5 Remove one of the eyepieces
and attach the centering telescope.
[e] Centering telescope (
☞
3.6.4)
▼
2-6 Place the analyzer into the optical path.
[f] FL turret (
☞
3.12.1)
When the analyzer block is attached, turn the FL turret
to place the analyzer block into the optical path.
[f] Analyzer slider (
☞
3.5.2)
When the FL turret is not used, insert the analyzer
slider into the analyzer slot and push it into the second
click position.
▼
2-7 Place the polarizer into the optical path.
[g]
Polarizer
(
☞
3.5.1)
▼
2-8 Align the white indicator on the polarizer with
the center of the graduations, and tighten the
clamp screw on the polarizer.
[g]
Polarizer
(
☞
3.5.1)
▼
2-9 Turn the eyepiece section of the centering
telescope
to focus on the pupil of the objective.
[e] Centering telescope (
☞
3.6.4)
▼
[a] [i]
[j]
[k]
[c]
[b]
[h]
[f]
[d]
[f]
[g]
[e]
