NEW BRUNSWICK SCIENTIFIC BioFlo 415, Руководство по эксплуатации

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59
New Brunswick Scientific User’s Guide
9
9
P
P
R
R
E
E
P
P
A
A
R
R
I
I
N
N
G
G
F
F
O
O
R
R
F
F
E
E
R
R
M
M
E
E
N
N
T
T
A
A
T
T
I
I
O
O
N
N
9.1 Systems Check
Prior to any fermentation run, make sure that all standard and optional systems
provided with the BioFlo 415 are operational:
•
Exhaust Heater
•
Agitation Control
•
pH Control
•
Temperature Control
•
Level 1 Control
•
DO Control
•
Gas flow Control
•
Level 2 Control
•
Pumps
Also review the following checklist:
1. Check to make sure that the drain valve is closed prior to filling the vessel.
2. Ensure that the fermentor is clean and ready for operation,
and that the vessel
jacket is primed
. This can be accomplished by setting the temperature setpoint
below the ambient temperature and putting the temperature loop in PID mode for 2
minutes. Then return to the growth temperature setpoint.
3. Confirm that all O-rings, gaskets, seals, filters and septa are in place, lightly coated
with silicone grease and in good condition.
4. Make sure that the RTD TEMPERATURE PROBE is securely in place in the
vessel’s thermowell.
CAUTION!
Proper installation of the RTD is essential to temperature control.
5. If sensors such as pH, DO, foam or level probes are to be used, make sure that they
have been properly inserted and are secure, and that the pH probe has been
calibrated.
6. Connect the pH and DO probe cables to the probes and connect the connectors to
the pH and DO connectors to the cabinet.
#
NOTE
:
The DO probe needs to be connected for a minimum of 6 hours, in order
to be polarized before it can be calibrated correctly.
7. Connect the foam and/or level probe cables to the appropriate probes at the
cabinet.