NEW BRUNSWICK SCIENTIFIC BioFlo 415, Руководство по эксплуатации

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79
New Brunswick Scientific User’s Guide
13.3 Pump Flow Rate & Calibration Methods
The pump will always run at the same speed, but the flow rate depends on the diameter
of the tubing you use. Table 9 provides the pump flow rates according to various
tubing diameters:
Table 9: Flow Rate per Tubing Size
Tubing Wall Thickness 1/16 inch (1.6mm)
Inside Diameter: inch (mm) 1/32 (0.8) 1/16 (1.6) 3/32 (2.4) 1/8 (3.2)
Flow ml/revolution
0.03 0.11 0.24 0.41
12 RPM Flow ml/minute
0.360 1.32 2.88 4.92
100 RPM Flow ml/minute
3.00 11.0 24.0 41.0
To calibrate any pump with the tubing you have selected:
1.
Load approximately three feet of the tubing into the pump head.
2. Set up a reservoir with water at the input end of the tubing and an empty graduated
cylinder, capable of measuring small quantities, at the output end of the tubing.
3.
Read this step completely before you do it:
with the input end of the tubing in
the water reservoir, prime the tubing line by pressing the pump’s Prime button, but
allow it to run only until liquid
starts
to flow into the tubing:
DO NOT
allow the
liquid to run into the graduated cylinder yet.
4.
If you are not using a scale, skip to Step 5.
If you are using a scale, place the
graduated cylinder (with the tubing) on the scale and press Zero on the scale.
5. In the Flow Rate pane of the PUMPS screen (
see Figure 43
) for that pump, press
the Calibrate button to open the Calibration pane (
see Figure 44
):
Figure 44: Calibrating the Pump Flow Rate
#
NOTE:
Calibration must be performed at operating setpoint.
6. Press your choice of
Run Time (15, 30 or 60
seconds); that button
will turn green.
7. Press Start. The button
will turn green and the
pump will start running.
9. Press the Set
button to save this
data to the PUMPS
screen.
8. When the Run Time has
elapsed, record the amount of
liquid accumulated in the
cylinder; enter that number
(or the number registered on
the scale) in the Amount
Pumped edit box.