Advanced protein features
Chapter 6
Zetasizer Nano accessories guide
Page 6-25
Measurement parameters table
When all the concentration values have been added into the table, use the
measurement parameters table on the right hand side to alter the result and
dynamic Debye plot.
The parameters are:
Molecular weight
The molecular weight of the sample (optional).
Partial specific volume
The partial specific volume of the sample (optional).
Second virial coefficient
The second virial coefficient of the sample (optional).
Solution Viscosity
The viscosity of the solution.
Temperature
The measurement temperature. This is displayed in both Celsius and Kelvin. If
either is completed, the other will be updated.
Saving the dynamic Debye plot
Click
Save
to save the parameters and other inputted data. To reviewed the saved
parameters at a later stage, click the
Load
button.
Copying the dynamic Debye plot
To paste the graph into another application (such as Microsoft Word or Excel)
select the
Copy
button.
Results area
When both the table data and sample parameters have been entered the results are
automatically calculated and shown alongside the graph. The results displayed are:
Diffusion coefficient
The calculated diffusion coefficient at zero concentration.
Stokes radius
The calculated radius at zero concentration.
DLS interaction paramete
r
The DLS interaction parameter (kD) is also known as the dynamic virial
coefficient and is related to the slope of the line on this plot.
Frictional coefficient
The frictional coefficient.
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