Malvern Zetasizer Nano Series, Руководство по аксессуарам

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Chapter 6 Advanced protein features
Page 6-22 MAN 0487
U
E
= electrophoretic mobility
Z = zeta potential
ε
= dielectric constant
Ƞ
= viscosity
F(
κ a
) = Henry's function.

Henry's function (f(
κ a
)) is defined as :
where:
ε
r
= dielectric constant
ε
o
= permittivity of free space (8.8542*10^-12 C/Vm)
a = hydrodynamic radies
κ
= inverse Debye length (1/nm)
NA = Avogadros' number (6.022*10^23 /mole)
K = Boltzmann's constant (1.38065*10^-23 m2kg/s2K)
T = temperature (K)
I = ionic strength (moles/L)
This calculator allows a more specific value other than the Smoluchowski or
Huckel estimates to be calculated and used for zeta potential measurements.
Protein charge
The protein charge calculation calculates protein charge from the measured
electrophoretic mobility and the hydrodynamic size. The charge is calculated from
the following equation:
where:
Z = calculated protein charge
ζ
= the zeta potential