3 Operation Procedures - Full Auto Protocols
35
QuickGene-Auto240L Operation Manual
3
■ Set the Reagents in the System
Set the reagents prepared in the previous section in the system as below:
Reagent Container and Required Reagent Quantity (for 2 mL of sample to treat)
Reagent
Reagent
Container
Setting
Position
No.
Quantity of
Use /1
sample
Other
Required
Quantity*
Required Quantity/1 Operation
8
Samples
16
Samples
24
Samples
EDB
Reagent
Container S
1
0.3 ml
1 ml
3.4 ml
5.8 ml
8.2 ml
LDB
Reagent
Container L
2
2.5 ml
10 ml
30 ml
50 ml
70 ml
Special Grade
Ethanol
(>99%)
Reagent
Container L
3
2.5 ml
10 ml
30 ml
50 ml
70 ml
WDB
(mixed with ethanol)
Wash Buffer
Bottle
4
19.5 ml
50 ml
206 ml
362 ml
518 ml
CDB
Reagent
Container S
5
0.5 ml
1 ml
5 ml
9 ml
13 ml
*The "Other Required Quantity" includes the quantity for filling the fluid feeding line in the system and the additional quantity for stable
suction of fluid.
(1)
Refer to the table above and split the required quantity of reagent in a reagent container for
QG-Auto240L.
Note:
After operation, the quantity of reagent included in the kit may fall short if the residual
reagent in the reagent container is disposed of.
The residual reagent in the reagent container should be preserved in a sealed container and
consumed as soon as possible.
(2)
Set the reagent containers S and L with reagent in them in the reagent container holder according
to the setting position numbers.
Set an empty container at the setting position numbers for reagent containers not to be used and
waste containers.
1
2
3
5
6
Waste Container 1 (Empty)
Waste Container 2 (Empty)
Setting Position 6 (Empty) For Additional Reagent
Содержание QuickGene-Auto240L
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