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3 Operation Procedures - Full Auto Protocols

QuickGene-Auto240L Operation Manual

3.2 Confirmation of Articles to be Prepared

Explanation regarding confirmation of prepared articles before conducting isolation work.

■QuickGene-Auto240L Main Unit and Accessories

Refer to "1.4 Checking of Packed Contents" and confirm that all are included.

■Other articles to prepare

The following articles shall be prepared.

NB: They are not included in the package of this system but must be

separately prepared.

◆

QuickGene DNA Whole Blood kit L (DB-L) for 48 Specimens/1 Kit

Cartridge x 48

Waste Tube

x 48

Reagents

x 1 set

◆

QuickGene-Auto240L Consumables Kit (QG-240L-CK) for 48 Specimens/1 Kit

Lysate Tube

x 48

10-mL Tip x 60

1.2-mL Tip

x 96

◆

1.5-mL Micro Tube, or 1.4-mL Matrix

Tube with 2D barcode

[Used as collection container (collection tube) for DNA]

◆

Special Grade Ethanol (>99%)

◆

Nuclease-free Water (Used for dissolution of pretreated enzyme (EDB) and for confirmation of

system functioning)

◆

Protective Gloves

◆

Safety Goggles

Содержание QuickGene-Auto240L

Страница 1: ...Automated Nucleic Acid Isolation System QuickGene Auto240L Ver 1 0 1 Operation Manual ...

Страница 2: ......

Страница 3: ...claimer The contents of this document are subject to change without prior notification Our company shall not be liable for Any violation of patent right of a third party or other rights through use of data described in document Any failure or damage caused by installation relocation remodeling maintenance or repair carried out by any party other than our company or other than a contractor designat...

Страница 4: ...re using this system SAFETY SYMBOLS Warning indicates the risk of death or serious injury if not avoided Warning indicates a risk of death or serious injury Biohazard indicates a risk of infection Caution indicates a risk of lighter or moderate injury or property damage Important Important indicates critical or prohibited actions Note Note indicates precautions instructions to abide by supplementa...

Страница 5: ...er of liquid on this system It may failure fire or electric shock If heat smoke or a strange odor is generated by the system remove the power plug from the outlet Any remodeling of this system without authorization by our company is prohibited Fire or electric shock may result Do not place any object on this system Do not drop any object onto or cause shock to the system A system failure or malfun...

Страница 6: ...kGene Auto240L Consumables Kit QG 240L CK NB These products are not included in this system package Please purchase separately Reagents Used for Other than Dedicated Kits For reagents used for other than a dedicated kit the work should be carried out in accordance with the safety precautions for handling and use of each reagent Use in a laboratory or work space suitable for isolation work Some rea...

Страница 7: ... the barcode reader or 2 dimensional code reader red light source during the sample ID reading operation Direct viewing at length may cause eye disorders Operation with a procedure other than indicated in the operation manual may lead to exposure to dangerous laser irradiation Laser Device Specification Specification Name Laser Sensor for detection of workpiece consumables 2 Dimensional Code Reade...

Страница 8: ...vi QuickGene Auto240L Operation Manual Warning Labels Warning caution instruction and obligation labels are affixed to this system ...

Страница 9: ...risk Warning Hot Surface Risk of burns do not touch immediately after operation Warning Laser Light Direct viewing of the laser light source may cause eye damage Do not directly view the laser light at length Warning UV Do not work for a long time in the proximity during UV irradiation Risk of damage to eyes and skin Caution Damage Do not apply force to the flap door by leaning or sitting on it or...

Страница 10: ...Gene Auto240L and QuickGene Consumables No liability is assumed for the results regardless of the purpose of use Validation of performance on samples shall be based on the judgment and responsibility of the user When using this system please thoroughly read this document and abide by the safety rules of your facility ...

Страница 11: ...ic Functions 2 3 Safety Mechanisms 2 4 Operation Panel Displaying Contents 2 5 Barcode Scanning Functions 2 6 Basic Specifications 2 7 Product Labeling 2 8 Implemented Protocols 3 How to Operate Full Automatic Protocol p 31 3 1 For Isolating Operation 3 2 Confirmation of Articles to be Prepared 3 3 Preparation of Reagents 3 4 Preparation of Consumables and Accessories 3 5 Preparation of Samples 3 ...

Страница 12: ...6 4 Setup of DNA Elution Buffer Volume 6 5 Parameters Setup Changed with the EXPERT Mode 7 Daily Inspections and Maintenance p 101 7 1 What to Do before Using the System 7 2 When System Not in Use for More than One Week 7 3 Cleaning of System Main Unit and Accessories 7 4 Replacement of Consumables 7 5 Cleaning of Wash Buffer Feeding Line 7 6 UV Ultraviolet Light Irradiating Function 8 Before Conc...

Страница 13: ...ocedures for setting up parameters are explained The procedures for daily inspection and maintenance are explained The handling procedures when you consider a failure has occurred are explained The warranties after sales services and precautions for transportation are explained 1 2 3 4 5 6 7 8 A 1 System Installation Procedures 2 Basic Functions 3 How to Operate Full Automatic Protocol 4 Operating...

Страница 14: ...xii QuickGene Auto240L Operation Manual ...

Страница 15: ...ved Watch your step carefully while operating Injury fire or electric may result from a damaged power cable Do not install this system in a location with intense vibration or at an unstable incline This may cause injury or failure Do not install this system in a location exposed to direct sunlight or in the proximity of a heating or cooling appliance This may cause a shortened lifetime or failure ...

Страница 16: ...system from a lower temperature to a warm place etc will generate water droplets in the system condensation and the isolation result will be adversely influenced A location where temperature and humidity within the specified ranges can be maintained Water faucets boilers coolers heaters etc should not be in proximity A location where strong magnetism motors transformers televisions speakers magnet...

Страница 17: ...1 System Installation Procedures 3 QuickGene Auto240L Operation Manual 1 1 2 How to Open the Package Refer to the separately attached QuickGene Auto240L unpacking instruction ...

Страница 18: ...em Installation Procedures 4 QuickGene Auto240L Operation Manual 1 1 3 How to Remove the Interior Packing Material and Fixtures Refer to the separately attached QuickGene Auto240L Fixture Removing Procedures ...

Страница 19: ...n the box If any item is missing please contact our customer consultation desk System Main Unit Operation Manual Power Cable Reagent Container Holder Sample Tip Holder Reagent Tip Holder Sample Holder 3 Cartridge Holder 3 Waste Tube Holder 3 Collection Tube Holder 3 Waste Container Wash Buffer Bottle Reagent Container S 3 Reagent Container L 4 UV Lamp Caution Note Use power cables holders containe...

Страница 20: ...ystem parts and their functions are explained below System Front View System Side View Operation Panel Power Switch Drawer Flap Door R Power Cable Sliding Door Flap Door L Right Side Left Side Inlet Power Cable USB Port 2 USB Port 1 LAN Port Serial Port 9 pin Power Cable Not Connected Power Cable Connected ...

Страница 21: ...Monitor Robot Unit Temperature Controller Monitor Barcode Reading Slot for Matrix Tubes Lysate Unit Cartridge Waste Tube Holder Slot Collection Tube Holder Slot Reagent Container Holder Slot Sample Tip Holder Slot Reagent Tip Holder Slot Sample Holder Slot Breaker Switch Waste Container Rack Wash Buffer Bottle Rack Wash Buffer Feed Pump ...

Страница 22: ...e Auto240L Operation Manual 1 Robot Unit Laser Sensor Rear For detection of Work Consumables Wash Buffer Nozzle Pressurizing Nozzle Pressurizing Packing Dispenser Drip Tray Level Detection Monitor Panel Tip Pressure Monitor Panel UV Lamp Syringe ...

Страница 23: ...minals and the lamp socket inserting position Insert the UV lamp and mount on the lamp socket 1 Insert the UV lamp along the vertical groove of the lamp socket to the end 2 Turn the UV lamp 90 to mount on the lamp socket Turn until a clicking sound is heard NB To remove the UV lamp follow the reverse order of above Lamp Socket Lamp Socket Side Align the center vertical lamp socket and the metal te...

Страница 24: ...edicated kit sample using the same procedure as for a normal isolating operation Refer to 3 How to Operate for separating operation 1 8 Precautions for Storage The precautions for storing this system are as follows Do not store this system while connected to the power source Do not store in an unstable location Store after disposal of used consumables and reagents Strong stains inside and outside ...

Страница 25: ...sed within the system The DNA is isolated in the collection tube on the dedicated holder set The basic functions of this system are explained as follows 2 1 Components Configuring the System The major components of this system are shown View of the System Work Area Inside Drawer 3 Operation Zone 1 Entry Zone 2 Holder Slot Zone 4 Power Switch 7 Robot Unit 8 Tip Injector 5 Isolation Unit 10 Consumab...

Страница 26: ...mixed in the set lysate tube and the preparation of lysate is processed On the upper surface of the unit an openable agitator cover is attached to prevent the lysate tube from ejecting 7 Robot Unit The activated dispenser pressurizing nozzles and wash buffer nozzles feed the fluid pressurize the cartridge and add the wash buffer Work progress detection with the laser sensor and ultraviolet light i...

Страница 27: ...d Full Auto Protocol is selected W BLOOD AUTO etc Screen Close CANCEL CANCEL A 8 Sample Quantity Input Screen BACK Sample ID reading setup OFF Setup in screen C 3 Full Auto Protocol is selected W BLOOD AUTO etc Collection ID reading setup 0FF Setup in screen C 3 COMPLETE Close Screen CANCEL Close Screen A 9 Sample Quantity Input Screen BACK OK OK After reading collection ID A 7 j Confirmation Mess...

Страница 28: ...ing Fluid Quantity Setup Screen DISP ELUTION BUFFER 1 C 3 Barcode ID Reading Function Setup Screen BACK C 5 Expert Mode Password Input Screen BACK BACK EXPERT MODE BARCODE READING Automatic start of operation whenthe result of checking is OK A Reagent Confirmation Screen BACK OK A 11 Operating Condition Setup Screen A 12 Checking Screen before Operation BACK START PRECHECK A 13 In Operation Screen...

Страница 29: ...RT Robot moves to pressuring nozzle packing maintenance position Power Switch OFF UV IRRADIATION BACK E 3 UV Irradiation Function Screen ERROR HISTORY BACK E 4 Error History Screen DATE AND TIME SETTING BACK E 5 Clock Setup Screen START UV Irradiation Start If when the power supply auto OFF setting is ON automatic power OFF INDIVIDUAL OPERATION BACK E 6 Individual Operation Screen A mode used for ...

Страница 30: ...red with a sensor and operations such as the isolation process are prohibited while a door is open Earth Leakage Breaker When an overcurrent occurs due to earth leakage or system malfunction the earth leakage breaker will shut off power The breaker condition can be checked on the breaker switch by opening the drawer of the system ON is seen while the breaker switch is in normal status and OFF is s...

Страница 31: ...e display will move to the user sign in screen by pressing OK 4 User Sign In Screen Displayed for user sign in Touch the white frame of USER ID and select a registered User ID Then touch the white frame of USER PASSWORD to display a ten key entry pad enter a password that is set up per user ID and press ENT Press SIGN IN to move on to the mode selection screen after matching the ID and password If...

Страница 32: ...on tube SEMI AUTO is a protocol for starting from the status of lysate preparation complete and CUSTOM is a spare protocol that allows the change of setups Press NEXT to move on to the next item and PREVIOUS to return to a previous item 9 Sample ID Check Screen Displayed when reading ID information and detecting number of samples while the sample ID barcode reading function is ON ID information is...

Страница 33: ...conditions Setup of Starting Position of Sample Tip Press RESET to start from the default position and set the value to 1 To start from another position press CHANGE to display a ten key entry pad enter an arbitrary value and press ENT to set Selection of Starting Level of Sample Suction Select BOTTOM for suction from the bottom level of the blood collection tube If SURFACE is selected the sample ...

Страница 34: ...r and Display of Details VIEW buttons are displayed at the left side of the screen Press VIEW to move on to the history ID information screen Press NEXT to move on to the next item press PREVIOUS to go back to the previous item Press DATA SAVE to move on to the operating history save screen Press BACK for returning to the mode select screen 19 History ID Information Screen A screen for confirmatio...

Страница 35: ...ten key entry pad Touch a white frame to display a ten key pad enter a value within an available range for setup and press ENT Change the value using buttons One press of the button will increase the setup by 0 01 and one press of the button will decrease the setup by 0 01 After setting a value press OK to complete the setup 24 Expert Mode Password Entering Screen A screen displayed when EXPERT MO...

Страница 36: ... of the button will increase the setup by 1 and one press of the button will decrease the setup by 1 After setting a value press START to start the wash pump feed the wash buffer into the wash buffer bottle and discharge into the waste container 28 UV ultraviolet light Irradiating Function Screen A screen displayed when UV IRRADIATION is pressed in the maintenance screen If the system power is to ...

Страница 37: ...n confirmation screen LYSATE DISPENSE Lysate fluid transferring function confirmation screen DISPENSE Dispenser function confirmation screen LASER SENSOR Laser sensor function confirmation screen PRESSURIZATION TEST Pressurizing test function confirmation screen 32 Ten Key Input Pop Up Screen Displayed when a value is to be entered in each screen 0 9 Enter values Enter decimal point Enter sign ENT...

Страница 38: ...em Readable ID Functions Barcode Reader for Blood Collection Tube Standard Sample ID Reads out the sample ID from the 1 dimensional barcode on the side surface of a sample tube such as a blood collection tube The ID information readout is checked against the location information A 1 A 2 for the collection tube Barcode Reader for Matrix Tube Option Collection ID Reads out the collection ID from the...

Страница 39: ...mm option Outline Dimensions 1280 mm W 720 mm D 990 mm H Mass Approx 300 kg System main unit only Operation Panel LC Touch Panel Rated Input Voltage Current 100 VAC 8 6 A 110 VAC 8 3 A 120 VAC 8 0 A 220 VAC 4 0 A 230 VAC 3 6 A 240 VAC 3 6 A Voltage Fluctuation 10 Phase Single Phase Frequency 50 60 Hz Environment for Use Indoor Use Overvoltage Category Transient overvoltage category II Applied Rate...

Страница 40: ...tifications Labels indicating the waste tube holder The alphabetical characters A C are the holder identifications 2 Labels indicating the sample holder The alphabetical characters A C are the holder identifications 3 Label indicating the reagent tip holder 4 Label indicating the sample tip holder 5 Label indicating reagent container setting position No 1 Label indicating reagent container setting...

Страница 41: ...ting slot for setting cartridge waste tube holder The alphabetical characters A C are the holder identifications 9 Label indicating slots for setting sample holder The alphabetical characters A C are the holder identifications 10 Label indicating slot for setting reagent tip holder FOR LYSATE and FOR REAGENT indicate the purpose for a tip to be set in a holder 11 Label indicating slot for setting ...

Страница 42: ...abel indicating Model Name Plate Affixed on the bottom right side surface of this system front side MODEL indicates the model number of this system INPUT indicates the input power voltage current and frequency S N indicates the serial number CE symbol marks are clearly stated ...

Страница 43: ...ding setups is not permitted W BLOOD DNA 1 0 mL FULL AUTO Full Auto Whole Blood 1 mL DNA PLASMA DNA 2 0 mL FULL AUTO Full Auto Plasma 2 mL DNA PLASMA DNA 1 0 mL FULL AUTO Full Auto Plasma 1 mL DNA W BLOOD DNA 2 0 mL SEMI AUTO Semi Auto Whole Blood 2 mL DNA W BLOOD DNA 1 0 mL SEMI AUTO Semi Auto Whole Blood 1 mL DNA PLASMA DNA 2 0 mL SEMI AUTO Semi Auto Plasma 2 mL DNA PLASMA DNA 1 0 mL SEMI AUTO S...

Страница 44: ...2 Basic Functions 30 QuickGene Auto240L Operation Manual 2 ...

Страница 45: ...or isolation work according to the work contents Take care not to cause contamination with sweat or sputum from preparation of sample until completion of isolation work 3 1 For Isolating Operation Abide by the following when conducting an isolating operation Abide by the setting order for accessories and consumables and set correctly Set the waste tubes cartridges and collection tubes in correct p...

Страница 46: ...ocation Waste Tube Holder Handle Cartridge Holder Collection Tube Holder Rule 3 The setting be without a vacancy and in order Rule 4 Use each holder according to the number of samples in the order of identification symbols A B C Use holder B or C when waste tubes cartridges and collection tubes are occupied in all positions in holder A or A and B Waste Tube Holder Handle Cartridge Holder Collectio...

Страница 47: ...repared NB They are not included in the package of this system but must be separately prepared QuickGene DNA Whole Blood kit L DB L for 48 Specimens 1 Kit Cartridge x 48 Waste Tube x 48 Reagents x 1 set QuickGene Auto240L Consumables Kit QG 240L CK for 48 Specimens 1 Kit Lysate Tube x 48 10 mL Tip x 60 1 2 mL Tip x 96 1 5 mL Micro Tube or 1 4 mL MatrixTM Tube with 2D barcode Used as collection con...

Страница 48: ...refrigerator 4 C which will provide stability for 2 months Preservation at 20 C will prolong the stable period for an enzyme but avoid repetition of thawing and freezing Note Use the pretreated enzyme EDB after completely dissolving in accordance with the following procedures Add 3 3 mL of nuclease free water set a lid and invert Leave for more than 30 minutes while occasionally agitating and conf...

Страница 49: ...er S 5 0 5 ml 1 ml 5 ml 9 ml 13 ml The Other Required Quantity includes the quantity for filling the fluid feeding line in the system and the additional quantity for stable suction of fluid 1 Refer to the table above and split the required quantity of reagent in a reagent container for QG Auto240L Note After operation the quantity of reagent included in the kit may fall short if the residual reage...

Страница 50: ...ner holder slot 4 Set awash buffer bottle in the wash buffer bottle rack in the drawer at setting position No 4 Note If the setting is not complete the inability to absorb wash buffer may influence the operation result Abide by the following and set a wash buffer bottle correctly The wash buffer bottle is set in such a way that the opening comes to the left side of the rack The ends of 2 inlet tub...

Страница 51: ...ure setting of tip rack with the holder without an uplift Confirm that equal to or more 1 2 mL tips than the samples are set from the starting position The tip use starting position can be changed in accordance with 3 8 Isolating Operation 2 Set 1 2ml tips and 10ml tips in a reagent tip holder Remove and set the required number of 1 2ml tips from the 1 2ml tip rack Note Set all reagent tips 1 2ml ...

Страница 52: ...ing close the cover and lock the locks in 3 places 5 Set the number of collection tubes equal to the number of samples in the collection tube holder Use the adapters according to the type of collection tubes Note When several holders are used set the collection tubes while paying attention to the holder identification symbols A C Handle Setting Order Handle Setting Order Handle Setting order Cartr...

Страница 53: ...ove chart To use the collection tube ID reading function the collection tube holder should not be set in this step but instead set in accordance with the instructions of the operation panel in 3 8 Isolating Operation Note The holder should be securely set in the slot until it contacts the stopper on the end Set a holder in the correct slot according to the manual and the identification label If a ...

Страница 54: ...ator cover of the lysate unit 3 Set the number of lysate tubes equal to the number of samples 4 After setting the lysate tubes close the agitator cover until a click is heard Note Be sure to securely close and fix the agitator cover until a click is heard If the fixing is insecure an error will occur during the system check before operation 5 Close the sliding doors ...

Страница 55: ... container in the waste container rack Securely set the waste container to fit the rack groove Note Be sure to correctly set an empty waste container included in the delivery The use of container not included in the delivery use of un emptied container or use with an erroneous setting may cause waste overflow 3 Close the drawer Waste Container Waste Container Rack ...

Страница 56: ...y inverting before use and start system operation as soon as possible Set the samples in the sample holder 1 Gently mix contents of the blood collection tube sample by inverting 2 Remove the lids of blood collection tubes 3 Set the blood collection tubes on the sample holder Use an adapter according to the type and size of the blood collection tube To use the sample blood collection tube ID readin...

Страница 57: ...To use the sample ID reading function the sample holder should not be set in this step but instead set in accordance with the instructions of the operation panel in 3 8 Isolating Operation Note The holder should be securely set in the slot until it contacts the stopper on the end of the system Set a holder in the correct slot according to the manual and the identification label If a holder is forc...

Страница 58: ...rm that the switches for 2 breakers are ON Check whether the breaker is shut off ON at the breaker switch is seen while in normal status and OFF at the breaker switch is seen when the circuit is shut off When the breaker is shut off refer to 8 1 Troubleshooting 3 Confirm that the sliding doors left right flap doors and drawer are closed The power can be turned ON with doors open but power will not...

Страница 59: ...to move on to the mode select screen 1 Confirm that the sliding doors left right flap doors and drawer are closed and press the power switch Refer to the previous section How to turn ON power Wait until the title is displayed 2 Press SYSTEM CHECK on the title screen and start the system check SYSTEM CHECK button will be enabled after the sliding doors and left right flap doors are locked 3 Press O...

Страница 60: ...lay a ten key entry pad enter a password which is set up per user ID and press ENT 6 Press SIGN IN 7 Each of the modes is executed from the mode select screen 1 AUTOMATED OPERATION Automatic Isolating Operation Mode Refer to 3 8 Isolating Operation 2 OPERATING HISTORY Operation History Mode Refer to 5 Operation History 3 PARAMETER SETUP Parameter Setup Mode Refer to 6 Parameter Set up Procedures 4...

Страница 61: ... mode select screen for how to move on to the user sign on screen 2 Touch the white frame of USER ID to display an alphabetical keyboard enter an arbitrary 5 10 digit alphanumeric string and press ENT Set up an alphanumeric string displayed as a user ID 3 Touch the white frame of PASSWORD to display an alphabet keypad enter a 5 10 digit number and press ENT Set up a password for the user ID 4 Re e...

Страница 62: ... move on to the mode select screen for how to move on to the user sign on screen 2 Touch the white frame of USER ID and select a registered User ID Select a User ID to delete 3 Touch the white frame of EXPERT PASSWORD to display a ten key pad enter a password for an expert and press ENT Contact our sales agent regarding the password for an expert 4 Press DELETE to delete the User ID ...

Страница 63: ...ing operation 1 Start Up of Automatic Isolating Operation Mode Selection of Protocol 1 Refer to 3 6 Start Up of System turn ON system power and move on to the mode select screen 2 Press AUTOMATED OPERATION 3 Press OK in the displayed pop up window Check that the waste container in the system drawer is empty and execute the automated operation mode 4 Press the full automatic protocol button to oper...

Страница 64: ...he barcode reader Direct viewing at length may cause eye disorders Note When setting a holder in the slot securely set until it contacts the stopper on the end When several holders are used pay attention to the holder identification symbols A C for setting A 1 The reading of complete samples will be displayed in reversed deep blue on the operation screen Check for the correctness of readout positi...

Страница 65: ...Sample Information A 7 Go to the next section 3 Enter Collection Information B When Sample ID Reading Function is OFF Note When the sample ID reading function is OFF refer to 3 5 Preparation of Samples and set the sample holders before turning the system power ON B 1 Press the numeric button corresponding to the number of set samples The pressed buttons will be indicated in reversed deep blue on t...

Страница 66: ...d with reading will be displayed in reversed green on the operation screen When the position of the read out collection tube and the previously entered sample setting position The laser light source categorized Class 1 is used for the 2D code reader Do not peer into the system while reading the collection ID and directly view the red light source Direct viewing at length may cause eye disorders No...

Страница 67: ...e holders are set in each slot and press COMPLETE When the positions of all the collection tubes and samples blood collection tubes coincide the COMPLETE button will be enabled C 6 The number of samples whose IDs are read out will be indicated in the pop up window If they are correct close all the doors and press OK C 7 Go to the next section 4 Confirmation of Reagent D When the collection ID read...

Страница 68: ...tion on the screen regarding the reagents to be used for the automatic isolating operation and confirm that the required quantity is set in the correct position 1 Press CHECK for a confirmed reagent If ALL is pressed all CHECK buttons are pressed at the same time 2 Press OK When all CHECK buttons are pressed the OK button will be enabled ...

Страница 69: ...based on the operation history the use starting position will be automatically set up accordingly Note Confirm whether the number of 1 2 mL tips is equal to or more than the number of samples and set from the use starting position If the sum of setup value and the number of samples automatically operated exceeds 96 reset the value of use starting position 81 65 49 33 17 1 82 66 50 34 18 2 83 67 51...

Страница 70: ... by detecting the surface using the differential pressure type fluid surface detecting function Note When BOTTOM mode is selected a pop up window to warn of overflow will appear After confirming that the sample quantity in the blood collection tube is not more than 3 mL press OK and return to the setup screen If the set sample quantity is more than 3 mL press BACK to go back to the mode select scr...

Страница 71: ...hecking Items Reference SAMPLE 3 5 Preparation of Samples REAGENT CONTAINER 3 3 Preparation of Reagents WASH BUFFER BOTTLE WASTE FLUID CONTAINER COLLECTION TUBE 3 4 Preparation of Consumables Accessories LYSATE TUBE DISPENSING TIP 1 2ml SAMPLE DISPENSING TIP 10ml LYSATE DISPENSING TIP 1 2ml REAGENT DISPENSING TIP 10ml REAGENT CARTRIDGE WASTE TUBE WASTE CONTAINER AGITATOR COVER Note When the isolat...

Страница 72: ...lue NG3 Clogged Cartridge Incomplete Isolation Gray No Sample Note Refer to 8 1 Troubleshooting or 8 2 Error Messages for incomplete isolation 2 The sample ID and collection ID information will be displayed by pressing ID INFO After confirmation of ID information press BACK and return to the operation result screen The ID INFO button will be enabled only when the ID reading function is ON When the...

Страница 73: ...Procedures Full Auto Protocols 59 QuickGene Auto240L Operation Manual 3 3 Press FINISH on the operation result screen 4 Press OK in the displayed pop up window 5 Press the system power switch and turn the power OFF ...

Страница 74: ...if the collection tube holder is tilted Take out while holding the handle of the collection tube holder with one hand and supporting the bottom surface of the holder with the other hand 2 Close the collection tube cap if a cap is used and remove the tube Note Securely close the cap If the collection ID reading function is OFF carefully conduct the removal and management of collection tubes paying ...

Страница 75: ...Daily Inspection and Maintenance and clean reagent containers and waste fluid containers Note Residual reagent in reagent container should be consumed as soon as possible Waste fluid should be disposed of in accordance with rules Sample Tip Holder Remaining tips should be stored in clean environment with no contamination Reagent Tip Holder Remaining tips should be stored in clean environment with ...

Страница 76: ...id Bottle Reagents remaining in cleaning fluid bottle should be sealed as is and stored When washing cleaning fluid bottle refer to 7 Daily Inspections and Maintenance Note When storing cleaning fluid bottles securely close caps Waste Container Dispose of waste in waste container When cleaning waste container refer to 7 Daily Inspections and Maintenance Biohazard Waste should be processed in accor...

Страница 77: ...n Continue the following operations as necessary Press RESUME to resume automatic operation Press the system power switch to shut the system power OFF Important The system will complete active movements before stopping when PAUSE is pressed Confirm a complete stop and then start the next operation Once power is shut down no resumption of the stopped movement is possible Even if operation is resume...

Страница 78: ...3 Operation Method Full Auto Protocol 64 QuickGene Auto240L Operation Manual 3 ...

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