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7 / Adjust conditions for the sample if necessary and record data
Once the system is stable and the resolution is good across the entire sample
size range, move onto the recording data stage. Use a lab book to record
the voltage, current, blockade magnitude and particle rate readings for
each recording, and always monitor the baseline current to track changes
in nanopore size. If the baseline current drops or becomes unstable or noisy,
or the particle rate drops, consult the troubleshooting guide to restore the
system stability.
Use the sample changeover method described on the previous page to
introduce the sample particles. To guarantee good resolution for your sample
particles AND high-quality data, you must choose a nanopore size that will
resolve your sample size distribution properly, AND choose a calibration
standard that matches your sample size:
Figure 14. Examples of poor and good resolution of sample particles on a nanopore.
Multiple calibration particles may be resolved on the same pore, but for best
results, choose one that gives you an average blockade magnitude of at least
0.25 nA (at a background current of 100 nA) at the stretch value that you can
resolve at least 90% of your polydisperse sample.
Use a smaller nanopore if you cannot properly resolve the sample at the
lowest recommended stretch (44.5 mm). Concentrate samples if the particle
rate is below 200 particles per minute at a pressure of 10 mbar.
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