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3 / Establish a baseline current:
The pore can be considered properly wetted when you can tap the fluid cell
cap and the current remains stable and does not jump or drift.
If the current is drifting, unstable, or still zero:
— Ensure that there is a voltage applied.
— Make sure the nanopore is stretched.
— Ensure that you are using the reverse pipetting technique.
— Mix the fluid in the upper fluid cell gently – without introducing more bubbles.
— Apply full pressure on the VPM and tap the fluid cell cap, to dislodge any
bubbles trapped in the aperture.
— Repeat the wetting process but use electrolyte instead of the wetting
solution and increase the stretch by 0.25 mm. Ensure that your
electrolyte contains between 0.03 and 0.1% surfactant.
4 / Estimate the size of the pore:
If the history of the pore is unknown, a quick size check can be easily
performed with PBS (with stretch = 47 mm, voltage = 0.1 V).
APPROXIMATE BASELINE
CURRENT AT 0.1 V (NA) NANOPORE SIZE (NP)
7-35 80
10-38 100
13-47 150
17-53 200
20-63 250
27-80 300
65-138 400
97-148 600
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