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KEY SKILLS FOR SAMPLE PREPARATION
To reduce the error associated with your concentration results, always use
calibrated pipettes and always use the smallest pipette for the job (e.g. use
a 2 µL pipette for dispensing 1 µL rather than a 10 µL pipette, and use a 200
µL pipette for dispensing 150 µL rather than a 1000 µL pipette). Always wipe
the outside of the pipette tip before dispensing, always dispense the smaller
volume into the larger volume, and pump the plunger 3-4 times before
discarding the tip.
1 /
Rotate the
thumbwheel to the
correct volume.
Attach pipette tip by
firmly pressing the
pipette down into the
top of a pipette tip in
the holder.
Immerse the pipette
tip into the fluid.
Smoothly release
the plunger to the
rest position.
Place tip at an angle in the
receiving vessel.
Smoothly depress plunger to the
first stop, then to the second stop.
With the plunger still depressed,
remove the pipette from the fluid.
Figure 10.Forward or standard pipetting should be used when diluting sample particles.
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