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41
Series 400 / 402 Gas Chromatograph Operating Manual GOW-MAC Instrument Co.
May 2017, Rev. 7
Chapter 8 - Experimental Run
8.1 Introduction
Since one of the purposes of the Series 400 GC is its use as an instructional instrument,
this chapter will, in general, run through a typical experiment.
The sample to be chromatographed is a mixture of
n
-heptane, tetrahydrofuran (THF),
2-butanone, and
n
-propanol in about equal parts. Such a mixture should be prepared.
8.2 Columns Needed
Column A:
8' x 1/8" 20% Carbowax 20M on Chromasorb P AW DMCS, 80/100 mesh
Column B:
8' x 1/8 20% DC-200 on Chromasorb P AW DMCS, 80/100 mesh
NOTE
&
If other columns are to be used, corresponding column and
injection port operating temperatures should be determined.
8.3 Gas Flow
!
IMPORTANT
Inert carrier gas
MUST
be fl owing through
both columns A
and B as well as sides A & B
of the TC detector.
THIS IS
EXTREMELY IMPORTANT and NECESSARY to preserve the
fi laments!
Failure to set fl ow rates on both sides causes oxidation and
destruction of the fi laments in the presence of oxygen.
A. POWER SWITCH
"OFF"
.
B. Turn Helium
"ON"
.
C. Check for leaks as described in Chapter 3.
D. Adjust fl ow to 80 mL/min in each column. Make sure to check fl ow for
BOTH
columns by
using the fl ow meter at the exit ports..
E. Disconnect fl owmeter.
8.4 Electrical
A.
DETECTOR CURRENT SWITCH
"OFF"
.
B.
BRIDGE CURRENT CONTROL
fully counterclockwise (CCW).
Содержание 400 Series
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