GE MOA 280i, Руководство по эксплуатации и обслуживанию

Страница: 123 / 211

GE Analytical Instruments ©2006
11-11
DLM 14291 Rev. A
reagents are contaminated. A blank should always be included to check for
contamination.
Cold ethanol precipitation
•
Rinse all glassware and tubes with deionized water to remove any
contamination.
•
Chill the required volume of ethanol to 0° C.
•
Place 0.5 mL of the sample in a 1.5 mL microcentrifuge tube, add 1 mL of cold
ethanol and vortex.
•
Let stand at 0° C for 30 minutes.
•
Centrifuge at ~14,000 RPM for 5 minutes.
•
Remove the supernatant for analysis. (Remember to account for 3-fold dilution
in the calculations).
Zinc Sulfate/Sodium Hydroxide precipitation
•
Rinse all glassware and tubes with deionized water to remove any residual
nitrate.
•
Add 200 µL of sample to microcentrifuge tube.
•
Add 400 µL of 0.5 N NaOH and 400 µL of 10% by weight aqueous ZnSO
4
.
•
Vortex for 30 seconds then let stand at room temperature for 15 minutes.
•
Centrifuge at ~14,000 RPM for 5 minutes.
•
Remove supernatant for analysis (remember to account for 5-fold dilution in
the calculations).