Action
Step
To change the display to show remaining Hrs, VhS or VhT:
5
Move the cursor under the Elapsed or ~ Remain field and use the Up or
Down arrow keys.
The temperature, voltage and current limits for the current step may be
adjusted directly on this screen as well.
R u n n i n g P r o t # 1 @ 2 0
˚
C
S t e p 1 o f 3 V h r s
S t e p V h r s E l a p s e d
5 0 0 V 4 0
µ
A / S t r i p 0 . 0 W
Pressing EDIT will access the step parameter screen. Pressing EDIT again
will return to the running screen. Use the Up arrow in the S1 to S3 fields to
access the Rehydration/IEF screen.
6
R u n n i n g P r o t #
S 1 G r a d 5 0 0 V 1 : 0 0 H r s
S 2 G r a d 2 0 0 0 V 1 : 0 0 H r s
S 3 S t e p 8 0 0 0 V 2 : 0 0 H r s
Note:
If you adjust the voltage during a gradient step the step will be converted to
Step for the remainder of the step.
As isoelectric focusing proceeds, the bromophenol blue tracking dye will
migrate toward the anode and leave the IPG strip. This generally occurs well
before focusing is complete
Note:
A colorless strip is no indication that IEF is complete.
7
If the tracking dye does not move, then no current is flowing across the strip.
Check that the strip holder electrodes are in contact with the electrode areas.
See the Troubleshooting section for additional suggestions.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
87
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
