Action
Step
Adjust the rehydration temperature by using the Left and Right arrow keys
to position the cursor. Use the Up and Down arrow keys to select the value
within the range of 15°C to 30°C.
5
The original factory programmed value is 20°C.
P r o t # 1 F i l e 1
R e h y d r a t e 1 0 : 0 0 H r @ 2 0
˚
C
I E F @ 2 0
˚
C 5 0
µ
A / S t r i p
1 S t r i p s 0 S t e p s
Note:
Maintaining the actual platform temperature at <18 °C or >25 °C may require
placement of the Ettan IPGphor 3 instrument in a location of lower or higher
than ambient temperature (e.g. a cold room).
It may be necessary to operate the instrument in an air conditioned room to
prevent condensation from collecting on the platform in humid environments
when the platform is run below 7°C ambient temperature.
The third line contains fields to set the platform temperature and current
limit for all isoelectric focusing steps.
6
Adjust the rehydration temperature by using the Left and Right arrow keys
to position the cursor. Use the Up and Down arrow keys to select the value
within the range of 15°C to 30°C.
To set the platform temperature use the Left and Right arrow keys to posi-
tion the cursor in the active °C field and use the Up and Down arrow keys
to select the value within the range of .
The original factory programmed IEF temperature is 20°C.
P r o t # 1 F i l e 1
R e h y d r a t e 1 0 : 0 0 H r @ 2 0
˚
C
I E F @ 2 0
˚
C 5 0
µ
A / S t r i p
1 S t r i p s 0 S t e p s
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
77
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
