Apply electrode pads prior to IEF
(optional)
Under certain conditions, such as prolonged focusing, water may migrate toward one
end of the strip, causing the other end to begin drying out. This effect can be minimized
by placing paper electrode pads between the IPG strip and each strip holder electrode
just before IEF. Electrode pads may also absorb ions that would otherwise accumulate
at the ends of the IPG strip and possibly interfere with the separation.
Follow the instructions below to apply electrode pads prior to IEF.
Action
Step
Cut two 3 mm wide electrode pads from a paper IEF electrode strip (18-1004-
40).
1
Position on a clean, flat surface such as a glass plate and soak with deionized
water. Remove excess water by blotting with tissue paper.
Note:
Electrode pads must be damp, not saturated or dripping.
2
Lift one end of the rehydrated IPG strip using forceps or tweezers. Position
an electrode pad over the electrode, then lower the strip back in place.
3
Repeat at the other end.
4
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
53
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
