Clean ceramic strip
holders/manifold
NOTICE
The ceramic strip holders and manifold are very brittle and fragile.
Take care in handling and do not subject to impacts.
The Ettan IPGphor 3 strip holders and manifold are made from a thermally conductive
aluminum oxide ceramic. They have been surface-treated to minimize adsorption and
interaction with proteins. The surface coating is mechanically robust and can withstand
temperatures of up to 300ºC, however it can be damaged by prolonged exposure to
extremes of pH.
Clean strip holders/manifold after each first-dimension IEF run. Do not let solutions dry
in the strip holder/manifold. Cleaning may be more effective if the strip holder/manifold
is first soaked a few hours or overnight in a solution of 2% to 5% IPGphor Strip Holder
Cleaning Solution in water. Strip holders and manifold may also be immersed in boiling
1% SDS.
NOTICE
You must use a neutral pH detergent, such as the IPGphor Strip
Holder Cleaning Solution, to remove residual protein from the strip
holders/manifold.
NOTICE
Fixed length strip holders and manifold may be baked, boiled or
autoclaved. DO NOT EXPOSE THEM TO STRONG ACIDS OR BASES,
INCLUDING ALKALINE DETERGENTS.
Instruction
Action
Step
First rinse off the strip holder/manifold. Use a mild liquid soap to remove
any residual IPG cover fluid.
1
Squeeze a few drops of IPGphor Strip Holder Cleaning Solution into the strip
holder/manifold slot. Use a toothbrush and vigorous agitation to clean the
strip holder/manifold.
2
104
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
6 Maintenance
6.1 Cleaning Ettan IPGphor 3
