Action
Step
In the Process Picture
4
•
Click the Sample inlet valve icon.
•
Select the position of the inlet to be filled. Start at the inlet position with
the highest number and end at the position with the lowest number or
the buffer position (assuming that the first sample to run is connected
to inlet 1 etc.).
Result:The sample inlet valve switches to the selected port.
Connect a 25 to 30 ml syringe to one of the purge valves of the pump heads
in the sample pump. Make sure that the syringe fits tightly into the purge
connector.
5
Open the purge valve by turning it counter-clockwise about three-quarters
of a turn. Draw slowly with the syringe until the sample just passes the
Sample inlet valve.
6
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
7
Repeat steps 4 to 7 for each sample inlet that is to be used in the method
run. The final sample or the buffer from the buffer position should be drawn
all the way through both pump heads into the syringe.
8
94
ÄKTA avant Operating Instructions 29101556 AB
4 Installation
4.5 Prime inlets and purge pump heads
4.5.2 Prime sample inlets and purge Sample Pump
