5.8
Prepare for a run at cold temperature
Introduction
To fit the ÄKTA avant instrument in a cold cabinet, the foldable door and pump cover
can be removed. For instructions, see ÄKTA avant User Manual. When using the instrument
in a cold room or cold cabinet, make sure to follow the precautions listed in the next
topic.
Precautions concerning runs in
a cold temperature
NOTICE
•
Avoid condensation. If ÄKTA avant is kept in a cold room, cold
cabinet or similar, keep it switched on in order to avoid conden-
sation.
•
Avoid overheating. If ÄKTA avant is kept in a cold cabinet and
the cold cabinet is switched off, make sure to switch off ÄKTA
avant and keep the cold cabinet open to avoid overheating.
•
Place the computer in room temperature. If the ÄKTA avant
instrument is placed in a cold room, use a cold room compat-
ible computer or place the computer outside the cold room
and use the Ethernet cable delivered with the instrument to
connect to the computer.
When the instrument is kept in a cold room, it is important to tighten all tubing
connectors, also the inlet manifold connectors. Otherwise air might get into
the flow path.
Note:
Make sure that the instrument, buffers and sample have had time to reach
the ambient temperature. When the instrument has reached the ambient
temperature, calibrate all pressure sensors.
Note:
When runs are performed in a cold cabinet, make sure to adjust the target
temperature of the built-in fraction collector temperature control function. The
target temperature is 20°C by default. Settings for the temperature control
function can be edited in the System Settings dialog box of System Control,
or in the Text Instructions pane in Method Editor.
Tip:
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ÄKTA avant Operating Instructions 29101556 AB
5 Prepare the system for a run
5.8 Prepare for a run at cold temperature
