Action
Step
Connect the syringe to the Injection Valve port Syr.
3
Load sample into the sample loop. To avoid sample loss due to siphoning,
leave the syringe in the port until the sample has been injected onto the
column during the run.
Tip:
It is recommended to overload the loop to make sure that the loop is com-
pletely filled. Excess of sample will leave the valve through port W1.
4
Sample application/injection
through a sample loop
A sample loop is manually filled with sample using a syringe connected to the Injection
Valve. During the method run, the sample is automatically injected onto the column. The
loop is emptied and washed out using buffer from the system pumps.
The total buffer volume to be used for emptying and washing the sample loop is set in
the Phase Properties tab of the Sample Application phase in the Empty loop with box.
134
ÄKTA avant Operating Instructions 29101556 AB
6 Run a method
6.2 Applying the sample
