Action
Step
In the Process Picture:
5
•
Click the System pumps icon.
•
Enter a low System flow (e.g., 0.2 ml/min).
•
Click Set flow rate.
Result: A system flow of 0.2 ml/min starts.
When buffer leaves the tubing on port 1A (if port 1A was chosen in the
method to be run) in a continuous mode and the top part of the column is
filled with buffer, connect the tubing to the top of the column.
6
ÄKTA avant Operating Instructions 29101556 AB
115
5 Prepare the system for a run
5.4 Connect a column
