Action
Step
In the Manual instructions dialog:
5
•
Select Flow path:Column position, and select the Position of the capillary
connected to the column valve. Click Insert.
•
Select Flow path:Sample inlet and select a Position. Click Insert.
•
Select Flow path:Injection valve and click Direct inject on the Position
menu. Click Insert.
•
Select Pumps:Sample flow and set the Flow rate to 25.0 ml/min. Click
Insert.
•
Click Execute.
Result: A sample flow of 25.0 ml/min starts.
Run the flow for 2 hours.
6
Discard the used buffer.
7
170
ÄKTA avant Operating Instructions 29101556 AB
7 Maintenance
7.3 Replace pump piston seal, O-ring, and rinse membrane of Pump P9-S
