Dionex PDA-100, Руководство оператора

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PDA-100 Photodiode Array Detector
3-18
Doc. 031644-02 9/03
Sample Wavelength
The PDA-100 measures absorbance over all wavelengths from 190 nm to 800 nm.
The deuterium lamp optimizes the UV range (190 nm to 380 nm). The tungsten
lamp optimizes the visible range (380 nm to 800 nm). Set the sample wavelength
at the wavelength with the absorbance maxima.
When the analyte wavelength maximas are known, the detector can run five
separate wavelengths without being required to collect spectra. Collecting
individual wavelengths instead of the spectra offers two advantages: it saves disk
space when 3D data is not required and it eliminates the need to perform
extractions for runs that do not require spectral data.
If little is known about the analytes in the sample, collect spectra over the full
wavelength range (using both lamps). Although this requires more disk space, it
provides complete information about the analytes and the spectra can be used to
determine peak identity, purity, and wavelength maxima.