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User’s Manual

PiezoDrill



Inertial Impact Drill

08572-M-01 (Rev. D)

Burleigh Instruments, Inc. 2002

IEZO

RILL



is a trademark of Burleigh Instruments, Inc.

Содержание PiezoDrill

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Страница 3: ...up 3 1 Filling the Injection Pipette with Fluorinert 3 1 Using Mercury in the Pipette 3 2 Attaching the PiezoDrill to the Microscope and the Micromanipulator 3 3 Critical Success Factors 3 4 PiezoDril...

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Страница 5: ...on the quality of the oocytes and many other factors that are beyond our control WARNING MERCURY WARNING USERS WHO CHOOSE TO USE MERCURY IN THE INJECTION PIPETTE WITH THE PIEZODRILL ARE STRONGLY CAUTI...

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Страница 7: ...rating the PiezoDrill and the following types of equipment Equipment type Equipment examples 1 Inverted microscope Leica DMIRB E Nikon TE200 300 Olympus IX70 Zeiss Axiovert 135 2 Injection pipette Hum...

Страница 8: ...actor for productive PiezoDrill utilization If the pipette is not shaped correctly the procedure will not be successful PiezoDrill pipettes manufactured to Burleigh specifications can be purchased fro...

Страница 9: ...optics at high magnification 300X 500X Any rough edges remaining after the pipette is broken should be gently removed by heat polishing using low heat The tip of the pipette does not need to be acid...

Страница 10: ...indicates that heavier fluids in the pipette work better with the PiezoDrill Some researchers have used mercury for PiezoDrill procedures However due to the significant health risks associated with ex...

Страница 11: ...e holding pipette should be large enough that some cytoplasm is held in place by the holding pipette We recommend an ID of 20 to 25 m because It provides more perivitelline PV space between the zona a...

Страница 12: ...Holders Hollow tube systems see Figure 4 allow the hydraulic fluid used to control the movement of material to pass through the rod and enter directly into the pipette via an O ring type of holder at...

Страница 13: ...the PiezoDrill and the syringe Solid Pipette Holders Solid pipette holders see Figure 5 require tubing from the syringe to be coupled directly to the pipette using Teflon or polyethylene tubing With...

Страница 14: ...that the syringe responds precisely to fingertip commands If pressure inadvertently increases during the procedure Fluorinert will be pushed out of the end of the pipette If pressure inadvertently dec...

Страница 15: ...s the Narishige B 8 ball joint see the PiezoDrill Operating Manual for assembly instructions The Narishige Adapter provides a stiffer connection that improves PiezoDrill performance We also make a Lei...

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Страница 17: ...the butt end of the pipette into the overhanging tubing 2 Firmly depress the syringe plunger allowing the Fluorinert to displace the air in the pipette thus filling the pipette Depending on the size...

Страница 18: ...se or recommend the use of mercury in the PiezoDrill Mercury should not be used with the PiezoDrill by any individuals or laboratories unwilling to bear the entire risk of exposure to mercury or unabl...

Страница 19: ...of mercury For this reason all of the ICSI dishes must be considered mercury contaminated All oil pipettes and all ICSI dishes must be disposed of as hazardous waste and labeled as containing mercury...

Страница 20: ...on the injection pipette side of the microscope check the following The pipette must be held tightly in the pipette holder The pipette holder must be tightly clamped in the PiezoDrill The pipette hold...

Страница 21: ...5 Duration 60 66 Frequency 2 1 Bandwidth 50 50 It is evident from these settings that the use of mercury in the pipette causes much stronger pulses and requires much lower settings The bandwidth setti...

Страница 22: ...ve one sharp pulse of the PiezoDrill with the following setting ADJUSTMENT SETTING Amplitude 35 Duration 90 Frequency 9 Bandwidth 100 Burleigh Instruments Inc does not suggest approve endorse or recom...

Страница 23: ...periodically during the procedure as well Please review the following steps in conjunction with viewing the Nuclear Transfer video on the enclosed CD 2 It is important to start with Fluorinert FC 77 c...

Страница 24: ...nterline vertically and horizontally If the embryo jumps around while drilling the injection pipette likely is not centered in the vertical plane Figure 9 Pre drilling position of the injection pipett...

Страница 25: ...r side of the embryo and activate pulse 2 to break the cytoplasmic membrane note in the video how the edge of the membrane next to the zona relaxes Using positive pressure inject the sperm or nucleus...

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Страница 27: ...use of Burleigh PiezoDrill customers 5 Recommended Procedure for a Successful Injection with Mercury in the Pipette The same 10 steps apply when using mercury in the pipette However only a small amoun...

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Страница 29: ...install the Narishige adapter in place of the ball joint 2 Be sure all manipulator and adapter connections are tight 3 Clamp the pipette holder on the manipulator as closely as possible to the PiezoD...

Страница 30: ...cy settings on the PiezoDrill Oocyte lyses during zona drilling 1 Use less suction 2 Create more PV space see Holding Pipette section 3 Lower the amplitude and or frequency settings Some degree of lys...

Страница 31: ...n Price List Burleigh Instruments Inc 2002 08572 M 01 Rev D A 1 The information contained in this document is privileged information and is intended for the personal and confidential use of Burleigh P...

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Страница 33: ...Burleigh Instruments Inc 7647 Main Street Fishers Victor New York 14564 8909 Tel 585 924 9355 Fax 585 924 9072 Email info burleigh com http www burleigh com PiezoDrill User s Manual 08572 M 01...

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