Burleigh PiezoDrill
Inertial Impact Drill User’s Manual
3 Setup
3-4
(08572-M-01 Rev D)
Burleigh Instruments, Inc. 2002
The information contained in this document is privileged information and
is intended for the personal and confidential use of Burleigh PiezoDrill customers.
Critical Success Factors
The PiezoDrill produces mechanical pulses that travel longitudinally along
the pipette and vibrate the tip. The tip vibrations aid in cutting (drilling)
through the zona pellucida and the oolemma in a less traumatic manner than
conventional procedures. One key to successful system operation is to
ensure that the pulses emanating from the PiezoDrill are accurately
transmitted through all the mechanical linkages in the system to the pipette
tip. If any connection in the system is loose, the frequencies can be altered
and the PiezoDrill may not operate effectively. Therefore, on the injection
pipette side of the microscope, check the following:
§
The pipette must be held tightly in the pipette holder.
§
The pipette holder must be tightly clamped in the PiezoDrill.
§
The pipette holder must be firmly attached to the micromanipulator,
ideally as close to the PiezoDrill as possible.
§
The micromanipulator must be securely attached to the microscope on a
sturdy platform/mounting system.
PiezoDrill Settings and Control Parameters
The PiezoDrill Operating Manual contains recommended beginning trial
settings for the amplifier for both zona and oolemma penetration. However,
the optimum settings will vary depending on the type of manipulator, the
type of fluid used in the pipette, the shape of the pipette and the nature of the
oocyte itself. In general, a stiffer manipulator will allow lower settings, as
will a heavier fluid in the pipette.
PiezoDrill Settings When Fluorinert is Used in the Pipette
ADJUSTMENT
ZONA SETTING
OOLEMMA SETTING
Amplitude
20
20
Duration
75
75
Frequency
10
2
Bandwidth
100
100
PiezoDrill Settings When Mercury is Used in the Pipette
