Burleigh PiezoDrill
Inertial Impact Drill User’s Manual
6 Troubleshooting Guide
6-2
(08572-M-01 Rev D)
Burleigh Instruments, Inc. 2002
The information contained in this document is privileged information and
is intended for the personal and confidential use of Burleigh PiezoDrill customers.
Oocyte lyses after oolemma
penetration.*
1.
Reduce suction in the pipette.
2.
Lower the amplitude and/or frequency
settings on the PiezoDrill.
Oocyte lyses during zona
drilling.
1.
Use less suction.
2.
Create more PV space (see Holding
Pipette section).
3.
Lower the amplitude and/or frequency
settings.
* Some degree of lysis may be expected, especially for mouse oocytes, due to the inherent
fragility of the oocytes. Human, non-human primate, rabbit, goat, sheep and pig oocytes
typically are much more robust than mouse oocytes for microinjection procedures. In
addition, larger pipette sizes often will lead to higher rates of lysis. Therefore, pipettes
should be sized to the smallest diameter suitable for the application.
