Table of Contents
Table of Contents ........................................................................................................................... 1-3
1. Introduction – Mercury Warning ............................................................................................ 1-1
2. Equipment and Materials ........................................................................................................ 2-1
Injection Pipettes........................................................................................................................ 2-2
Purchasing Injection Pipettes........................................................................................... 2-2
Manufacturing Injection Pipettes ...................................................................................... 2-2
Fluids Used in the Injection Pipette.................................................................................. 2-4
Holding Pipettes......................................................................................................................... 2-5
Manufacture or Purchase of Holding Pipettes ................................................................. 2-5
Pipette Holders .......................................................................................................................... 2-6
Hollow Tube Pipette Holders............................................................................................ 2-6
Solid Pipette Holders........................................................................................................ 2-7
Microinjector............................................................................................................................... 2-8
The Importance of a Good Microinjector.......................................................................... 2-8
Micromanipulators...................................................................................................................... 2-9
3. Setup ......................................................................................................................................... 3-1
Filling the Injection Pipette with Fluorinert ................................................................................. 3-1
Using Mercury in the Pipette...................................................................................................... 3-2
Attaching the PiezoDrill to the Microscope and the Micromanipulator ...................................... 3-3
Critical Success Factors ............................................................................................................ 3-4
PiezoDrill Settings and Control Parameters .............................................................................. 3-4
PiezoDrill Settings When Fluorinert is Used in the Pipette .............................................. 3-4
PiezoDrill Settings When Mercury is Used in the Pipette ................................................ 3-4
Using the PiezoDrill to Break Off the Sperm Tail............................................................. 3-6
Foot Pedal Control of Two Independently Adjustable Channels ..................................... 3-6
4. Recommended Procedure for a Successful Injection with Fluorinert in the Pipette........ 4-1
5. Recommended Procedure for a Successful Injection with Mercury in the Pipette .......... 5-1
6. Troubleshooting Guide ........................................................................................................... 6-1
