Burleigh PiezoDrill
Inertial Impact Drill User’s Manual
3 Setup
Burleigh Instruments, Inc. 2002 (08572-M-01 Rev D)
3-1
The information contained in this document is privileged information and
is intended for the personal and confidential use of Burleigh PiezoDrill customers.
3. Setup
Filling the Injection Pipette with Fluorinert
A very effective way of filling a pipette with Fluorinert is to use a 5 or 10 ml
syringe fitted with a blunted 20 gauge needle. Blunting the needle (breaking
off the end) produces a better flow of material.
1.
Cover the needle with a piece of polyethylene tubing
1
so that about 1
centimeter of tubing extends over the end of the needle. Remove all air
bubbles from the syringe and tubing then carefully fit the butt end of the
pipette into the overhanging tubing.
2.
Firmly depress the syringe plunger, allowing the Fluorinert to displace
the air in the pipette, thus filling the pipette. Depending on the size of
the opening at the tip, which can be very small for most applications, this
procedure may take a minute or so.
3.
Disconnect the pipette from the tubing, use positive pressure in the
microinjector to push the oil in that system to the end of the tubing,
attach it to the pipette holder of choice. It is important that there are
minimal air bubbles trapped between the oil and the Fluorinert when the
pipette is coupled to the pipette holder in order to achieve good control
during the micromanipulation procedures outlined in Section 4.
Figure 6 illustrates the proper technique for filling the injection pipette with
Fluorinert.
1 Suggested tubing: Tygon
Microbore, 0.38 mm ID, 2.29 mm OD, 0.76 mm wall, Cole-
Parmer cat. no. EW-06418-03
