Creating Programs
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The Extend Time Option
This programming option progressively extends an incubation step with each subsequent
cycle. This is typically used during an extension step, to allow for diminishing activity of
an enzyme, or to allow an enzyme to do its job among an ever-increasing quantity of
product.
Assume, for example, that we required a 60°C step with a 1 minute incubation and
that we wished to increase the incubation time by 5 seconds per cycle. In the
Temperature Step window, you would implement the following:
•
Enter 60°C in the Temperature field.
•
Enter 1 in the Time:Min field.
•
Select Extend Time.
•
Select <Set Parameters> (for the Extend Time option).
The Extend Time window appears.
•
Select Increase.
•
Position the cursor over the by __ seconds per cycle field and select.
•
Enter 5 in the field.
•
Select <OK>.
This program step, incorporating a per cycle increase in incubation time,
would appear as follows:
Incubate at 60°C for 00:01:00
Increase by 5.0 seconds every cycle
This step is not included in our cycle sequencing protocol, so we continue
with our next addition.
