DNA Engine Tetrad 2 Thermal Cycler Operations Manual
5-8
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Example Program
Let’s start with an example. Assume you have the necessary components for a 30-cycle
amplification reaction, and you have calculated the annealing temperature of your oligonu-
cleotide to be 60°C. Please note that we recommend using 92°C as the default
denaturation temperature during cycling steps. The resulting raw program you write may
look something like this:
Raw program:
1.
92°C for 30 seconds
2.
60°C for 3 minutes
3.
92°C for 30 seconds
4.
60°C for 3 minutes
5.
92°C for 30 seconds
6.
60°C for 3 minutes
[continues for a total of 60 lines]
The Goto Option
At 60 lines, our program is large, unwieldy and would take time to input. At step 3,
repetition can be reduced with the addition of a goto statement:
Raw program:
1.
92°C for 30 seconds
2.
60°C for 3 minutes
3.
Goto step 1, 29 more times
4.
END
One of the most important factors in the program writing process is identifying repetitive
steps. These can then be enclosed in a goto loop as shown above.
