DNA Engine Tetrad 2 Thermal Cycler Operations Manual
5-30
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•
Similarly, enter 1 in the Time:Min field.
Additionally, we want the cycler to beep after reaching the target temperature.
•
Select Beep on Target.
At this point, in the Temperature Step window, all selections have been made
according to our protocol.
•
Select <OK>.
In the advanced programming window, the program listing now appears as
follows:
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100°C
1. Incubate at 94°C for 00:01:00
Beep on Target
End
The program, if run now, would ramp to 94°C for 1 minute and then end. Let’s
continue to enter steps and build our program.
The first temperature step in the cycling portion of our program incubates at
92°C for 30 seconds. We would also like the cycler to beep after reaching this
temperature.
•
Position the cursor over the appropriate insertion point, if not highlighted
already, and select.
Note:
The appropriate insertion point would be selected by highlighting the
first step. Insertion will occur AFTER this step.
•
Select <Temp>.
•
Position the cursor over the Temperature field and select.
•
Enter 92 from the numeric keypad into the field.
•
Similarly, enter 30 in the Time:Sec field.
Additionally, we want the cycler to beep after reaching the target temperature.
•
Select Beep on Target.
At this point, in the Temperature Step window, all selections have been made
according to our protocol.
•
Select <OK>.
In the advanced programming window, the program listing now appears as
follows:
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100°C
