BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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8 | Helios Gene Gun System
Section 3 Product Description
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Spermidine (for example, Sigma, catalog numbers S-0266 or S-4139)
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Calcium chloride (CaCl
2
)
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Plasmid DNA (for most applications, this should be at a concentration of ~1 µg/µl)
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Plasmid DNA of high purity suitable for the Helios Gene Gun can be obtained through use of any of
Bio-Rad’s Quantum Prep Plasmid Prep Kits. See Section 9, Product Information, on page 41.
Section 3
Product Description
Packing List
The Helios Gene Gun System (see Figures 3 and 4) is shipped with the following components. If items are
missing or damaged, contact your local Bio-Rad office.
Helios Gene Gun Kit
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Instruction manual
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Warranty card (please complete and return)
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Helios Gene Gun
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5 cartridge holders
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5 barrel O-rings
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5 barrel liners (four plus one installed in gene gun)
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9 volt battery
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Cartridge extractor tool
Helium hose assembly
Helium regulator
Tubing cutter and 10 razor blades
Tubing Prep Station (see Figure 4)
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Tubing prep unit (base, tubing support cylinder, and power cord)
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Nitrogen hose (12' (~4 m), Nalgene Tubing 8000-0030, 3/16" ID, 5/16" OD)
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3/16" barb-to-male luer fitting
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10 cc syringe sleeve