BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 10 Appendices
44 | Helios Gene Gun System
Replacing the O-Rings and Barrel Liner on the Helios Gene Gun
The Helios Gene Gun has three user-serviceable parts: the barrel liner and two O-rings (Figure 22). The
barrel liner screws into the barrel of the Gene Gun. It is a molded plastic piece with the correct shape to
permit optimum spread and penetration of the microcarriers at the target site. It may be autoclaved between
experiments or replaced.
Fig. 22. Location of barrel liner and O-rings in the gene gun. The barrel (cover) is removed for visual clarity but is not required for barrel
liner removal/insertion.
To remove the barrel liner:
Hold the exposed part of the barrel liner (the spacer) and turn counterclockwise 4–5 turns (see Figure 5).
To insert the barrel liner:
1. Remove the cartridge holder from the gene gun.
2. Place the barrel liner into the gene gun and turn clockwise until the threads just bind.
Do not overtighten.
The O-rings function to keep the helium from expanding around the tube in the firing position in the cartridge
holder. One O-ring is inserted at the end of the barrel liner that makes contact with the cartridge holder; the
other O-ring surrounds the exit port of the main valve. Both O-rings fit in grooves with center supports.
To replace the O-ring on the end of the barrel liner:
1. Remove the barrel liner.
2. Remove the old O-ring from the barrel liner.
3. Place a new O-ring in the groove and press it in gently. Do not use grease or adhesive.
To replace the O-ring around the exit port of the main valve:
1. Using a pin or syringe needle, poke the O-ring from the edge to pop it out. Be careful not to scratch the
surface of the valve.
2. Place a new O-ring in the groove and press it in gently. Do not use grease or adhesive.
Barrel liner
O-rings