BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

Страница: 21 / 60

Section 4 Inserting the Battery into the Helios Gene Gun
Helios Gene Gun System | 17
■
10 cc syringes (2)
■
10 cc syringe sleeve
■
1/8" barb-to-male luer fittings (2)
■
1/8" barb-to-female luer fittings (2)
■
Scissors, user supplied
Fig. 11. Components and controls on the Tubing Prep Station, fully assembled.
Procedure
1. Place an O-ring in the stainless steel end of the tubing support cylinder next to the spur gear.
2. Insert the end of the tubing support cylinder with the spring into the hole in the support bar on the base
of the Tubing Prep Station. Push the tubing support cylinder to compress the spring, then slide the end
of the tubing support cylinder containing the O-ring into the support on the motor housing.
(
Note: Carry the Tubing Station by the base, not by the tubing support cylinder.)
3. Cut a 4–5" piece of syringe adapter tubing; attach each end to the barb of the 1/8" barb-to-male luer
fittings. Attach one of the male luer fittings to the female luer on the top of the flowmeter. Attach the
other male luer fitting to the female luer on the side of the motor housing on the Tubing Prep Station.
4. Cut a 12–13" piece of syringe adapter tubing; attach one end of the tubing to the barb of a 1/8"
barbtofemale luer fitting; attach the female luer to a 10 cc syringe. This syringe and tubing will be used
to load the DNA/microcarrier suspension into the GoldCoat Tubing (Guide to Operation in Section 5).