BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 5 Operation of the Helios Gene Gun System
Helios Gene Gun System | 27
(see Replacing the O-Rings and Barrel Liner on the Helios Gene Gun, in Section 10).
1. Insert an empty cartridge holder into the Helios Gene Gun as follows:
a. Move the cylinder lock on the gene gun so it is latched in the forward position and the barrel pin
does not protrude behind the barrel (Figure 12).
b. Unlatch the push bar by pulling it outward (Figure 12).
c. Pull back and hold the cylinder advance lever to retract the inner barrel sleeve into the gun barrel
(Figure 13).
d. Place the empty cartridge holder into the gene gun with the position 12 label facing up and the
numbered side of the cartridge holder facing the exit nozzle of the barrel (Figure 12).
e. When the cartridge holder is in its correct position, the knob on the backside of the cartridge holder
will slip into the notch on the barrel plate and the cartridge holder will be flush with the barrel plate.
f. Release the cylinder advance lever; the O-ring on the inner barrel sleeve should hold the cartridge
holder in position.
g. Unlatch the cylinder lock; it should snap into position and the barrel pin should be inserted into the
center hole in the cartridge holder.
h. Push the push bar in; it should snap back into position and engage the cartridge holder in one of the
deep crevices (see Figure 14).
Fig. 13. Inserting a cartridge holder into the gene gun. Pulling the cylinder advance lever in moves the barrel forward to increase the space for
inserting the cartridge holder behind the barrel liner. The cartridge holder is inserted with the labeled position number 12 (visible at the top) in line with
the center of the gene gun (see also Figure 12).