BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 10 Appendices
Helios Gene Gun System | 45
Replacement of Razor Blade on and Disassembly of the Tubing Cutter
The cutting edge of the tubing cutter is a standard single-edge razor blade. Because cutting is done only on
one end of the blade at once, it can be rotated after the first end becomes dull. The first sign of a dull blade
will be incomplete cutting of two adjacent cartridges. Front refers to the side of the tubing cutter with the
tubing channels. Right, left, and back refer to the sides of the tubing cutter when the user faces the front of
the unit (see Figure 23).
1. Loosen the red locking knob on the back of the tubing cutter by turning it approximately one full turn
until the lock block on the rear of the cutting arm is free to move.
2. Move the lock block to the right to free the razor blade retaining screw on the right side.
3. Lift up on the right side of the razor blade until it can be lifted free of the tubing cutter.
Replacing the O-Ring on the Tubing Prep Station
The Tubing Prep Station has two user-serviceable parts: O-rings at the end of the tubing support cylinder
nearest the motor. If the O-rings are worn, nitrogen may leak around them and the amount of nitrogen
indicated on the flowmeter will not be passing through the tubing. Under normal use, the lifetime of the
O-rings should be longer than 1 year. The O-rings may be changed as follows (see Figure 23):
1. Remove the tubing support cylinder by pushing it to the right to contract the spring on the support bar,
then raise the left side up and away from the base of the unit.
2. The outer O-ring should be located in the slot at the end of the tubing support cylinder, although it may
remain in the support on the motor housing. It may be removed with a fingernail or a blunt object, such
as a toothpick. If worn, replace the O-ring with a new one.
3. The inner O-ring is at the end of the tubing support cylinder next to the stem. To replace it, remove the
stainless steel stem at the end of the tubing support cylinder by loosening the hex nut on the lower
surface of the tubing support cylinder one-eighth turn using a 5/64" Allen wrench. The stem and spur
gear can be pulled out of the tubing support cylinder. Remove the O-ring from the slot in the stem with a
fingernail or blunt object and replace if worn. Insert the stem into the end of the tubing support cylinder;
press gently on the end to hold the O-ring in place and tighten the hex nut. Replace the tubing support
cylinder in the base of the Tubing Prep Station.
Fig. 23. Replacing the O-rings on the Tubing Prep Station. The O-rings are located in the tubing support cylinder on the side next to the motor
housing. One O-ring (A) fits into a slot on the outside edge of the stem at the end of the tubing support cylinder. The other O-ring (B) is on the inside
surface of the stem and can be removed only after loosening the hex nut and removing the stem from the tubing support cylinder.
Stem
Hex nut Spur gear
O-ring
B
O-ring
A