BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 4 Inserting the Battery into the Helios Gene Gun
Helios Gene Gun System | 15
Connecting the Helios Gene Gun to a Helium Source
Refer to Safety Information, in Section 1, and Identification of System Components and Controls, in Section
3, prior to system installation.
Helium Pressure Regulator Installation
Components needed
■
Pressure regulator for helium cylinder (with pressure relief valve, check valve, and female Swagelok
Quick-Connect Fitting), provided with unit (Figure 10)
■
Helium cylinder of grade 4.5 or higher (minimum 99.995% pure); maximum pressure of 2,600 psi
user supplied.
■
One 1/8" open end wrench or a 10" or 12" adjustable wrench, user supplied
Note: The regulator is intended for use only with helium gas with a maximum pressure of 2,600 psi. The
outlet on pressurized helium cylinders used in the U.S. is compatible with the fitting supplied on the pressure
regulator with the Helios Gene Gun System (CGA 580, female fitting). Outside the U.S., contact your local
Bio-Rad office for information regarding the proper cylinder/regulator fitting in your area.
Procedure
1. Secure the cylinder in a floor stand or to a wall or lab bench with a strap so it will not tip or fall
during use.
2. Inspect the cylinder valve for dirt, dust, oil, grease, or damaged threads. Remove dust and dirt with a
clean cloth. Do not attach the regulator if you determine that the valve port is damaged or cannot be
cleaned. Inform your gas supplier of this condition and request a replacement cylinder.
3. Clear the valve port of any foreign matter by standing to the side of the cylinder and quickly opening and
closing the cylinder valve.
4. Attach the regulator to the cylinder valve and tighten securely with a 1 1/8" open end wrench or a
10" or 12" adjustable wrench.
Attaching the Helios Gene Gun to the Helium Regulator
Components needed
■
Helium regulator attached to a helium cylinder
■
Helium hose assembly
■
Helios Gene Gun
Procedure
1. Insert the stem of the Swagelok Quick-Connect Fitting on the helium hose into the opening in the
body of the Swagelok Quick-Connect Fitting on the helium regulator and push until it clicks. The
helium hose will be locked into the helium regulator (see Figure 10).
Note: If the helium regulator has
been pressurized, the stem and body will not lock. Turning the regulator valve counterclockwise will
depressurize the system.