BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Helios Gene Gun System | 11
Section 3 Product Description
Gene Gun Controls Description
Cylinder lock Controls movement of the barrel pin. The cylinder lock is spring-loaded; its natural position is in the backward (locked)
position so the barrel pin is inserted in the hole in the cartridge holder; this keeps the cartridge holder in its proper
position for firing. Moving the cylinder lock forward disengages the barrel pin from the cartridge holder to permit
removing the cartridge holder from the gun. Moving the cylinder lock forward and to the right latches the cylinder lock
to permit removal of the cartridge holder; however, to prevent damage to the O-rings, the cartridge holder should be
removed only after compressing the cylinder advance lever (see below).
Safety interlock switch Switch that must be held down to permit the trigger button to be operational. Once this switch is depressed, the trigger
button is functional for 30 sec; the LED ARMED display flashes quickly during this time. If the trigger button is not
pressed within the alloted time, this safety interlock switch must be released and pressed again to reactivate the trigger
button.
Trigger button Controls the flow of helium gas through the gene gun. This switch activates the solenoid, opening the main valve for
~40 msec and permitting helium to enter the cartridge and barrel. The trigger button is active for only 30 sec after the
safety interlock switch is depressed.
Cylinder advance lever A multifunctional lever that is spring-activated by pulling the lever backwards. When inserting or removing a cartridge
holder, pull back and hold in the cylinder advance lever; this moves the barrel liner forward to provide additional room
for maneuvering the cartridge holder. After discharging the microcarriers from one cartridge, pull back and release the
cylinder advance lever; this ratchets the cartridge holder, bringing the next cartridge into firing position. The number
visible on the very top of the cartridge rim indicates the active sample position.
Push bar
A metal bar that ratchets the cartridge holder from one position to the next when the cylinder advance ever is pressed.
Moving this bar to the left (outward) when inserting a cartridge holder provides additional room for maneuvering the
cartridge holder
.
LED display An 11-light display. The display is normally off; inserting a cartridge holder in the gene gun and advancing to position 1
activates the display. The left-most 7 LEDs act to indicate charging and ready status of the gun. After each firing of the
gun and at reset, the CHARGING LEDs turn on in bar graph fashion, left to right, throughout the 5 sec charging time.
Once the gene gun is fully charged, the CHARGED LED will flash, indicating the safety interlock switch can be pressed.
On pressing the safety interlock, the ARMED LEDs flash sequentially. When the trigger is pressed during the 30 sec
armed period, the gun fires and the FIRED LED turns on for 1 sec. The change bar graph then operates as described.
The last light indicates battery status: good battery (steady green light) or low battery (flashing red light). The gene gun
can be fired when the green light is illuminated. If the battery is low, neither the safety interlock switch nor the trigger
button is active and an alarm will beep three times every 15 sec.
Tubing Prep Station
A tool for coating the DNA/microcarrier solution onto the inner wall of GoldCoat Tubing. See Figure 6.
Tubing Prep
Unit Controls
Description
3-position switch
Located on the motor housing and controls rotation of the tubing support cylinder. At position (I), the tubing support cylinder
turns continuously at 30 revolutions per min (rpm). At position (II), the tubing support cylinder rotates only while the switch is
depressed. At position (O), no rotation occurs and the unit is off.
Flowmeter Registers the rate of nitrogen flow in liters per min (LPM) into the tubing support cylinder. The valve on the flowmeter is used
to control the rate of nitrogen flow.
Tubing support
cylinder
A 28" aluminum cylinder with an opening on the right side leading to a channel that holds the GoldCoat Tubing. The left side
of the channel has a replaceable O-ring into which the tubing must be inserted. The tubing support cylinder can be removed
by pushing it to the right/left to compress the spring that holds it in position.