BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 5 Operation of the Helios Gene Gun System
Helios Gene Gun System | 31
1. Place the cartridge holder on a flat surface with the numbered edge facing up.
2. Starting with position 1, load up to 12 cartridges into the cartridge holder (Figure 17). We recommend
loading only one type of DNA per cylinder to avoid possible confusion.
3. Invert the cartridge holder and push the cartridges against a flat surface so that they are flush with the
numbered side of the cylinder.
4. Insert the loaded cartridge holder into the Helios Gene Gun as described above. When the LED on the
back of the gene gun indicates that the first cartridge is in firing position (Figure 15) the device is ready
to deliver DNA.
DNA delivery to target tissue (see Section 6 for suggestions on preparing mammalian target cells)
1. Touch the target area with the spacer so that the spacer is flush and the gene gun is perpendicular to
the target surface. Activate the safety interlock switch and press the trigger button to deliver the DNA/
microcarriers to the target.
2. Ratchet to the next cartridge by pulling in and releasing the cylinder advance lever. After approximately
5 sec, the gene gun is ready to deliver the next cartridge.
3. After all tubes have been discharged, remove the cartridge holder as described below.
Removing Used Cartridges, Depressurization, and Shutdown
Materials
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Cartridge extractor tool (supplied)
Removing Cartridges from the Cartridge Holder
1. Hold the cartridge holder containing the cartridges to be extracted with the back side (the side with the
knob in the center) facing up.
2. Insert the long prong of the Cartridge Extractor Tool into one of the bores of the cartridge holder; turn
the Cartridge Extractor Tool until all of the prongs mesh with the bores in the cartridge holder.
3. Push the prongs of the tool into the cartridge holder until the cartridges are ejected.
Depressurizing and Shutting Down the Helios Gene Gun
1. After discharging the last cartridge, turn off the helium pressure to the system by closing the valve on
the helium tank.
2. Turn the regulator valve counterclockwise until both the high and low pressure gauges on the helium
regulator register 0 psi. Several increase/decrease adjustments on the regulator may be necessary.
Listen for venting to ensure complete depressurization. The system is now depressurized and can be
safely disassembled.
3. Disconnect the helium hose from the regulator by pulling the sleeve on the Swagelok Quick-Connect
Coupling toward the helium hose and pulling the fittings apart.
4. Disconnect the helium hose from the gene gun by pulling the sleeve on the Swagelok Quick-Connect
Coupling toward the gene gun and pulling the fitting apart. ( Warning: For safety, do not leave the Helios
Gene Gun unattended while attached to the helium regulator.)