BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 10 Appendices
52 | Helios Gene Gun System
Quick Guide to Operation
Before the Bombardment
1. Coat microcarriers with DNA, load into tubes, and prepare cartridges prior to day of experiment.
2. Check helium supply (50 psi in excess of desired rupture pressure).
3. Clean and/or sterilize the gene gun, tube holders, and barrel liner as appropriate.
4. Connect the gene gun to a helium source.
5. Activate the gene gun: turn on the flow of helium to the desired pressure and with an empty cartridge
holder in place, make 2–3 pre-shots by engaging the safety interlock and firing the trigger.
Firing the Device
1. Load cartridges into the cartridge holder and place in gene gun.
2. Prepare/position target cells for bombardment.
3. Bombard sample: engage safety interlock and press the firing trigger
After the Bombardment
1. Remove cartridge holder from gene gun.
2. Remove cartridges from cartridge holder.
3. Turn off the helium pressure to the system.
4. Turn the regulator value counterclockwise to de-ressurize the system.
5. Disconnect the helium hose and gene gun.
Go to
bio-rad.com/Helios/training
for additional Helios Gene Gun training and overview information,
including instrument setup, microcarrier and cartridge preparation, and instrument operation.