BIO RAD Helios Gene Gun System, Инструкция по эксплуатации

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Section 5 Operation of the Helios Gene Gun System
26 | Helios Gene Gun System
Particle Delivery Using the Helios Gene Gun
This section describes the procedure for preparing the Helios Gene Gun for firing, discharging the device,
loading cartridges into the cartridge holder, and delivering DNA to target cells. (
Warning: Use the Helios
Gene Gun in a well ventilated area.)
Materials
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Supplied
Helios Gene Gun
Barrel liner (see Cleaning and Sterilizing the Helios Gene Gun, in Section 10, on sterilization)
Cartridge holder
Helium hose
Helium regulator (see Connecting the Helios Gene Gun to a Helium Source, in Section 4)
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To be Supplied by User
Helium tank (see Requirements for System Operation, in Section 2)
Ear protection
Cartridges with coated microcarriers (from Preparation of System Components Prior to Bombardment,
in Section 5)
Activating the Helios Gene Gun for Gene Delivery
When first setting up the system and prior to discharging the Gene Gun into a target, it is important to
pressurize the helium hose and the internal reservoirs of the gun with the correct helium pressure. This is
accomplished by discharging the device prior to delivering loaded tubes to the target cells. It is important to
have a cartridge holder in place during these “pre-shots” to keep the O-rings from being blown out
Fig. 12. Positioning the cylinder lock and the push bar in preparation for loading a cartridge holder into the Gene Gun. The cylinder lock has
been pushed forward and into the slot on the right to move the locking pin away from the opening occupied by the cartridge holder. Likewise, the push
bar has been pulled to the left to increase the space for inserting the cartridge holder.