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SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS

All samples must be incubated within 6 hours of sampling.

Dispense a sterile absorbent pad into a sterile petri dish, and saturate the pad with prepared
broth

Loosen the graduated filter funnel, and remove from the base support.

Sterilise the forceps and allow to cool. Using these forceps, place a sterile membrane onto
the glass membrane support, grid side up. If the membrane tears or becomes contaminated,
discard it and use a new one.

Lock the membrane in place by screwing the filter funnel down into position.

Pour the water sample into the filter funnel up to the 100 ml graduation.

Содержание WSL25 Pro

Страница 1: ...AquaSafe WSL25 Pro Water Safety Laboratory Instruction Manual ...

Страница 2: ...s Section 1 Introduction Section 2 Kit Contents Section 3 Single Incubator Instructions Section 4 Membrane Filtration Instructions Section 5 HydroTest HT1000 Instructions Section 6 Multi parameter Instructions ...

Страница 3: ...ntegrated single chamber digital incubator complete with all accessories and consumables for carrying out 300 tests for Faecal and Total Coliforms Photometer kit complete with all accessories and consumables for 300 tests of Ammonia Free Total Chlorine Nitrate and Nitrite Multi parameter electrochemical meter kit complete with electrodes and calibration solutions for testing pH conductivity and TD...

Страница 4: ...Section 2 Kit Contents 1 4 5 9 Under Accessories Box 7 8 2 3 6 10 11 i h g f e d c c c c b a k l l m j ...

Страница 5: ...0 Pack b 1 Dilution Tube c 4 Sample Collection Bottles with Dechlorination Tablets d 1 5ml Sterile Syringe e 1 30ml Dropper Bottle for Methanol f 1 Membrane Filtration Unit including water beaker sample beaker measuring funnel sintered glass disk 1 silicone gasket g 1 Sterile Membrane Filters 100 Pack h 1 Forceps i 1 Vacuum Tube j 1 Eyeglass k 1 Vacuum Pump l 2 Sterilised Water for Membrane Lauryl...

Страница 6: ...be for HydroCheck 1 Thermal Probe for HydroCheck 1 pH 4 01 Buffer Solution 1 pH 7 00 Buffer Solution 1 EC 1413µS Calibration Solution Separate Box Qty Description 3 Ammonia Reagents 100 Pack 3 Chlorine Free Total Reagents 100 Pack 3 Nitrite Reagents 100 Pack 3 Nitrate Reagents 100 Pack 3 Arsenic Test Kit 100 Tests 4 Sterilised Water for Membrane Lauryl Sulfate Broth Preparation 2 Sterile Membrane ...

Страница 7: ...Section 3 AquaSafe Micro biological Incubator Instruction Manual ...

Страница 8: ...15 3 3 2 Start Screen Figure 1 1 16 3 3 3 37 C Program Fig 1 2 17 3 3 4 44 C Program Fig1 3 18 3 3 5 User defined Program Fig 1 4 19 3 3 6 Resetting the timer Fig 1 5 19 3 3 7 Incubation cycle time setting Fig 1 6 20 3 3 8 User defined temperature setting Fig1 7 20 3 3 9 Calibration Fig 1 8 21 4 0 Care and Maintenance 22 4 1 General 22 4 2 Cleaning 22 4 3 Maintenance 23 5 0 Technical Specification...

Страница 9: ...d with the supplied 54mm x 3 5mm aluminium petri dishes which are suitable for 47mm membrane filter pads but the incubator can also be used with 55mm pre prepared plastic petri dishes The incubator is supplied with 25 Petri dishes as standard but has the capacity for more due to the Petri Lok system The incubator has the option to run 37 C 44 C and user defined temperature profiles for periods of ...

Страница 10: ...ot become depressed during transit covering the recessed plate with rigid card should achieve this 2 1 Docking Station The Incubator is mounted on a docking station built into the carry case which incorporates the rechargeable battery pack and allows the incubator to be run from the batteries during transportation It is also possible to use the incubator on a bench independent of the dock if desir...

Страница 11: ... if absolutely necessary 2 3 Battery Charging It is recommended to charge the battery fully prior to use To do this remove the power supply unit from the kit and connect it to the charging connection on the rear of the carry case screwing firmly into position Plug the other end into a 90 240V AC outlet then switch on the unit via the on off switch on the rear of the case The indicator on the charg...

Страница 12: ...e used cable available separately Note The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o replacement should be used Replacements are available from any Trace2o approved representative No attempt should be made to charge or power the equipment other than via the approved Trace2o equipment 2 5 Fuse The docking station contains a fuse to protect th...

Страница 13: ...isting to the left and then removing the top To re fit align the arrow with either of the two slots in the top of the rack push down and twist lock into place The incubator has a power connection socket on the right hand side of the unit and three switches on the top which control all functions of the incubator The incubator can be run from a power supply in the range 12 24 Volts All information i...

Страница 14: ...ailure conditions If Fig 1 0 is displayed and the incubator was in mode 2 then the previous incubation cycle can be restarted from where it was left by pressing restart This will be discussed later The screen will be displayed for 60 seconds before defaulting to the restart condition Pressing the right hand switch will reset the incubator ready to set up and start a new incubation cycle and the sc...

Страница 15: ...g sequence 20 9 C 24 00 OFF MEM 10 5v Select Option Restart New Run 20 9 C 24 00 37 MEM 10 5v 20 9 C 24 00 44 MEM 10 5v 20 9 C 24 00 U MEM 10 5v 20 9 C 24 00 New SEL 10 5v 20 9 C 24 00 Cal ADJ 10 5v 20 9 C 24 00 U ADJ 10 5v 20 9 C 24 00 Tim ADJ 10 5v Fig 1 0 Fig 1 1 Fig 1 2 Fig 1 3 Fig 1 4 Fig 1 5 Fig 1 6 Fig 1 7 Fig 1 8 ...

Страница 16: ...re 1 1 This screen shows the current status of the incubator In the state shown the incubator is in idle mode and not running Current Temperature Time Remaining Incubator Status Battery Voltage Mode 20 9 C 24 00 OFF MEM 10 5v ...

Страница 17: ...d hold the middle switch again for 5 seconds The display will return to the one shown in Fig 1 2 Once the chamber has reached the pre set temperature the timer will start to countdown If for any reason the temperature should drop such as power failure the timer will auto pause until the temperature is back to the pre set temperature It should also be noted that if there is a power failure the time...

Страница 18: ... is described in section 3 3 7 Pressing and holding the middle switch for 5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater To stop or pause the incubation cycle press and hold the middle switch again for 5 seconds The display will return to the one shown in Fig 1 2...

Страница 19: ... middle switch again for 5 seconds The display will return to the one shown in Fig 1 2 3 3 6 Resetting the timer Fig 1 5 In this mode pressing the middle switch will display the following screen which will flash rapidly to warn the user this will reset the timer and the elapsed time data will be lost The time remaining is displayed in the top left corner This can be reset to the programmed default...

Страница 20: ...e middle switch to save the settings The display will return to the one shown in Fig 1 1 3 3 8 User defined temperature setting Fig1 7 In this mode the user defined incubation temperature can be adjusted to a temperature between 20 C and 50 C To adjust the temperature press the middle switch The screen will change to Use the left switch to decrease the temperature and the right switch to increase ...

Страница 21: ...r Compare the displayed temperature on the incubator screen to that shown on the thermometer If there is a difference 0 2 C the calibration should be adjusted as follows Exit the run mode and step through the screens until the calibration screen is displayed Press the middle switch to enter calibration mode The following screen will be displayed Adjust the temperature up or down using the left and...

Страница 22: ...wet conditions and must not be submersed in water The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned as soon as possible The Aluminium chamber can be cleaned with Alcohol soap or solvent based cleaners Do not use abrasive or chlorine based cleaners The Petri Lok rack can be steam sterilised if required but it is not recommended to steam sterilise...

Страница 23: ...he rechargeable lead acid batteries may be necessary If so these must be replaced with batteries of the same type as specified in section 5 1 and replaced as a pair Should any fault occur please contact the Trace2o Technical Team who will be more than happy to advise you 5 0 Technical Specification 5 1 Docking station Power supply Input Max 12 28V DC 48W via supplied Power supply unit or vehicle c...

Страница 24: ...nd Assistance Trace2o hope that the AquaSafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the AquaSafe incubator is covered by Trace2o Ltd s standard Guarantee terms and conditions available via email or via download from www trace2o com In the event that any technical assistance is required the Trace2o Customer Service department will ...

Страница 25: ...Section 4 AquaSafe Membrane Filtration Instruction Manual ...

Страница 26: ... 29 SECTION 3 PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD 30 SECTION 4 SAMPLING 31 SECTION 5 USE OF BACTERIOLOGICAL MEDIA 33 SECTION 6 ASEPTIC PROCEDURES 34 SECTION 7 PROCESSING SAMPLES FOR COLIFORM ANALYSIS 36 SECTION 8 COUNTING COLIFORMS AND RECORDING THE RESULT 39 SECTION 9 SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION 1 ...

Страница 27: ...LTRATION APPARATUS 1 Hand Vacuum Pistol Pump 2 Filtrate Flask Waste Beaker 3 Sampling Cup and cable 4 Graduated Aluminium Funnel 5 Membrane Support Holder 6 Sealing Gaskets 7 Glass Sintered Disc Membrane Support 3 4 1 5 6 7 2 ...

Страница 28: ...r and press into place Place the sintered disc smooth side facing upwards into the centre of the gasket Push the other two gaskets into place around the sintered support disc The graduated funnel screws clockwise into position Do not over tighten the funnel ...

Страница 29: ...r cleansing agent pour the prepared medium carefully into the bottles Replace bottle lids but leave them slightly loose do not fully tighten Sterilise in an autoclave at 121 C for 10 minutes or place bottles in a pressure cooker and maintain in steam at pressure for 15 minutes Remove the bottles allow them to cool to room temperature fully tighten the tops and then store in a cool dark place When ...

Страница 30: ...ange to green to indicate moisture penetration Tear open the sachet and remove the silica gel with the forceps If the silica gel is green discard the sachet without using Open a bottle of Sterilised water for broth preparation and carefully pour the entire contents of the sachet into the bottle Replace the bottle lid tightly and vigorously shake the mixture to ensure that the powder is fully disso...

Страница 31: ...mple collection bottles contain dechlorination tablets N B If the sample is analysed immediately on site then it is not necessary to dechlorinate the water Dechlorination Kit The dechlorination tablets are pre placed inside each sample collection bottle Simply pour the sample from the sampling cup into the sample collection bottle cap and shake thoroughly to ensure thorough dissolving of the table...

Страница 32: ...until the water cools to its original temperature Fill the prepared bacteriological sampling bottle leave a slight air gap at the top and seal the lid tightly Dip Sample Sterilise the sampling cup by igniting 1ml of methanol alcohol in the cup Allow to cool Rinse the alcohol from the cup with water from the sample source Immerse the cup into the water source to obtain the sample Pour into the prep...

Страница 33: ... in a water bath for 15 minutes Clean empty media bottles thoroughly before re use Any residues should be washed out with hot water cleaned with a little detergent a small brush can be used if required rinsed several times in clean water dried and stored in a clean environment with the lids lightly attached The MLSB solution may be applied to the pads up to 6 hours before sampling if the pads are ...

Страница 34: ...r ignite the methanol TAKE CARE AROUND THE NAKED FLAME Allow the methanol to burn for a few seconds Whilst the methanol is still burning invert the filtration unit and carefully place inside the sample cup Wait for at least 20 minutes to ensure that the sample cup and filtration unit are sterile Methanol burns anaerobically to form formaldehyde gas which reaches all areas of the filtration apparat...

Страница 35: ... is necessary to dispense pads in the field great care must be taken not to contaminate either the pad dispenser or the cartridge As soon as a cartridge is finished a new one should be attached to the dispenser Do not leave the dispenser unattached for any length of time If no pad dispenser is available use sterilised forceps Before handling a membrane filter with the forceps the forceps should be...

Страница 36: ... the graduated filter funnel and remove from the base support Sterilise the forceps and allow to cool Using these forceps place a sterile membrane onto the glass membrane support grid side up If the membrane tears or becomes contaminated discard it and use a new one Lock the membrane in place by screwing the filter funnel down into position Pour the water sample into the filter funnel up to the 10...

Страница 37: ...Connect the hand vacuum pump to the filtration unit base and pump in a controlled fashion to suck the water sample through the membrane ...

Страница 38: ...ple number place date time etc Place the petri dish into the PetriLok cartridge Repeat the process for all the samples and then place the filled rack into the incubator It is important to note that when the last sample has been processed a resuscitation period of at least one hour but not more than four hours must be observed before incubating This allows any physiologically stressed coliforms to ...

Страница 39: ...thin a few minutes of removing from the incubator as the colours are liable to change on cooling and standing Ignore those colonies that are not yellow e g pink transparent colonies Once the number of yellow colonies has been determined and assuming that 100 mL of sample was filtered this number of colonies equals the number of coliforms per 100 mL Where samples were incubated at 35 C the count is...

Страница 40: ...ds 10 faecal coliforms per 100 mL action is urgently required Raw Waters For source waters and partially treated waters including those which are ground water derived it can be useful to adjust the sample volume in order to obtain faecal coliform counts in the optimum range 10 200 It may also be useful to process more than one quantity on the first occasion a particular water source is sampled In ...

Страница 41: ...Section 5 HydroTest Photometer HT1000 ...

Страница 42: ...thod Information F1 10 2 3 4 Chemical Species Information 11 2 3 5 Differentiation 11 2 3 6 Performing Zero 11 2 3 7 Performing Tests 12 2 3 8 Changing Chemical Species 12 2 3 9 Storing Results 13 2 3 10 Printing Results T2o PC Link Optional 13 2 3 11 Performing Additional Measurements 14 2 3 12 Selecting a New Method 14 2 3 13 Measuring Absorbance 15 2 4 Photometer Settings Table of Mode Function...

Страница 43: ...al Functions Service 48 2 5 Data Transfer 48 2 5 1 Data Printing 48 2 5 2 Data Transfer to a Personal Computer 48 3 0 Enclosure 49 3 1 Unpacking 49 3 2 Technical Data 49 3 3 Abbreviations 50 3 4 Troubleshooting 52 3 4 1 Operating Messages in the Display Error Display 52 3 4 2 General 54 ...

Страница 44: ... Standard Methods for water analysis This instruction manual outlines the functions and operations of the photometric instrument only Instructions specific to each test are included with the reagent packs as purchased from Trace2o Ltd The instrument has been designed and optimised to work solely with Trace2o reagents A complete list of available parameters is included in Appendix 1 0 The instrumen...

Страница 45: ...battery changeover time exceeds 2 minutes all the data and settings stored on the device are lost It is recommended to have the replacement batteries easily accessible to ensure quick changeover 2 1 3 Replacement of Batteries Ensure to read Chapter 2 1 2 before replacing batteries See Chapter 2 1 4 for an annotated diagram of the instrument 1 Switch the instrument off 2 If necessary remove vial fr...

Страница 46: ...B Battery Compartment Cover C Notch D Batteries 4 x AA LR6 E Seal Ring F Instrument Back CAUTION To ensure that the instrument is waterproof Seal ring E must be in position Battery compartment cover B must be fixed with the four screws ...

Страница 47: ...y description in the text if key available Function 2 key description in the text if key available Function 3 key description in text if key available Displaying date and time user countdown Storing of displayed test result Menu of photometer settings and further functions Moving cursor up or down Performing Test Returning to selection of methods or previous menu Confirming Performing Zero Decimal...

Страница 48: ... entering a new value The entry comprises two digits each for minutes and seconds E g 2 minutes 0 seconds Shift 0 2 0 0 Confirm with key The display shows Start countdown with key After countdown has finished the photometer reverts to the previous display automatically 2 2 4 Display Backlight Press Shift F1 keys to turn the display backlight on or off The backlight is switched off automatically du...

Страница 49: ...ys to select the required method a Press arrow key or to select the desired method from the displayed list Confirm with key b Enter method number directly e g Shift 8 0 to select Bromine 2 3 3 Method Information F1 Use F1 key to switch between the compact and the detailed list for the method selection Example Line 1 Method number Method name Line 2 Range Line 3 Kind of reagent Line 4 Vial Lines 5 ...

Страница 50: ...ome methods e g Chlorine The photometer then requires the type of determination a Press arrow key or to select the required determination Confirm with key 2 3 6 Performing Zero The display shows Prepare a clean vial as describe in the relevant application note and place the vial in the sample chamber ensuring that the triangular markings on the vial and HT1000 are aligned Press ZERO key The displa...

Страница 51: ...When the test result is displayed press the arrow key or Example 320 Phosphate LR T 320 Phosphate LR T 320 Phosphate LR T 0 05 4 mg l PO4 0 02 1 3 mg l P 0 04 3 mg l P2O5 1 00 mg l PO4 0 33 mg l P 0 75 mg l P2O5 If the species of a test result is changed the displayed range is adjusted automatically For an already stored result it is not possible to change the chemical species The last displayed c...

Страница 52: ...are free the display shows Clear the memory as soon as possible see Chapter 2 4 3 Delete Stored Results If memory capacity is full it is not possible to save additional test results 2 3 10Printing Results T2o PC Link Optional If the PC Link module is switched on see Chapter 2 5 Data Transfer and the printer is connected it is possible to print out the test results without saving beforehand Press F...

Страница 53: ...firm with TEST key Or press ZERO key to perform a new zero calibration The display shows 2 3 12Selecting a New Method Press ESC key to return to method selection Or enter the required method number directly e g Shift 1 6 0 for CyA TEST Cyanuric acid Confirm with key Zero accepted prepare Test press TEST prepare Zero press ZERO ...

Страница 54: ... list or by entering the corresponding method number directly The display shows e g 430 nm Always carry out zeroing using a filled vial e g deionised water The display shows Confirm with TEST key The display shows e g TIP To ensure complete reaction times the user countdown may be helpful Chapter 2 2 3 User Countdown 900 mAbs 430 nm 2600mAbs 2600mAbs Prepare Zero Press ZERO Zero accepted prepare T...

Страница 55: ...ults of one selected method 23 Printing params 29 Set printing options 24 Storage 30 Display all stored results 25 Stor date 31 Display only results of selected time period 25 Stor code No 32 Display only results of selected code No range 26 Stor method 33 Display only results of one selected method 27 Delete data 34 Delete all stored results 28 Calibration 40 Special method calibration 29 User ca...

Страница 56: ...r Saturation Index water balance 48 Temperature 71 Select C or F for Langelier mode 50 LCD contrast 80 Set the display contrast 51 LCD brightness 81 Set the display brightness 51 System Info 91 Information about the instrument e g current software version 53 2 4 1 Instrument Basic Settings 1 Selecting a Language Press MODE Shift 1 0 keys Confirm with key The display shows Press arrow key or to sel...

Страница 57: ...y beep off Press Shift 1 keys to switch the key beep on Confirm with key Note In the case of methods with reaction periods the acoustic signal still sounds during the last 10 seconds of the countdown even if the key beep is switched off ...

Страница 58: ...function It is possible to switch the countdown off for all methods Press MODE Shift 1 3 keys Confirm with key The display shows Press Shift 0 keys to switch the countdown off Press Shift 1 keys to switch the countdown on Confirm with key Note It is possible to interrupt the working countdown by pressing the key application e g serial analysis The user countdown is also available if the countdown ...

Страница 59: ... periods an acoustic signal still sounds during the last 10 seconds of the countdown even if the key beep is switched off 2 4 2 Printing of Stored Results Printing All Results Press MODE Shift 2 0 keys Confirm with key The display shows Press for printing out all stored results The display shows e g After printing the photometer goes back to Mode Menu automatically Note It is possible to cancel th...

Страница 60: ... date range are printed After printing the photometer goes back to mode menu automatically Note It is possible to cancel the entry by pressing ESC If you want to print only results of one day enter the same day twice to determine the period Printing Results of a Selected Code No Range Press MODE Shift 2 2 keys Confirm with key The display shows Enter numeric code number up to 6 places for the firs...

Страница 61: ...nting Results of a Selected Method Press MODE Shift 2 3 keys Confirm with key The display shows Select the required method from the displayed list or enter the method number directly Confirm with key In case of differentiated methods select the required kind of determination and confirm with key The display shows e g Press key and all stored results of the selected method are printed After printin...

Страница 62: ...on with ESC key 2 4 3 Recall Delete Stored Results Recall All Stored Results Press MODE Shift 3 0 keys Confirm with key The display shows The stored data sets are displayed in chronological order starting with the latest stored test results Press key and all stored results are displayed Press F3 key to print the displayed result Press F2 key to print all results Printing params 2 Baud rate cancel ...

Страница 63: ... and day for the first day of the required period e g 19 May 2009 Shift 0 9 0 5 1 9 Confirm with key The display shows Press key and all stored results in the selected date range are displayed Press F3 key to print the displayed result Press F2 key to print all results End with ESC key Note It is possible to cancel the entry by pressing ESC If you want to recall only results from one day enter the...

Страница 64: ...ss key and all stored results in the selected date range are displayed Press F3 key to print the displayed result Press F2 key to print all results End with ESC key Note It is possible to cancel the entry by pressing ESC If you want to recall only results of one code number enter the same code number twice If you want to recall all results without code no Code no is 0 enter zero 0 twice Storage so...

Страница 65: ... In case of differentiated methods select the required kind of determination and confirm with key The display shows e g Press key and all stored results of the selected method are printed Press F3 key to print the displayed result Press F2 key to print all results End with ESC key Storage 20 Acid demand T 35 Alkalinity p T 30 Alkalinity tot T Test No Storage method 20 Acid demand T Start cancel ES...

Страница 66: ... 4 Calibration Calcium Hardness Method 191 Calibration of a Method Blank Press MODE Shift 4 0 keys Confirm with key The display shows Press Shift 1 keys The display shows 1 Fill a clean vial 24 mm Ø with exactly 10 ml of deionised water close tightly with the cap 2 Place the vial in the sample chamber making sure that the triangular marks on the vial and instrument are aligned 3 Press ZERO key Del...

Страница 67: ... Press TEST key The batch related method blank is saved Press key to go back to mode menu Notes 1 If a new batch of CALCIO tablets is used a calibration of the method blank has to be performed to optimise the results 2 Deionised or tap water 3 If no water free of calcium is available these ions can be masked by using EDTA Preparation Add 50 mg spatula tipful of EDTA to 100 ml of water and dissolve...

Страница 68: ...ml SPADNS reagent solution to the water sample Caution vial is filled up to the top 6 Close the vial rightly with the cap and swirl gently several times to mix the contents 7 Place the vial in the sample chamber making sure that the triangular marks on the vial and instrument are aligned 8 Pres TEST key The display shows 9 Remove the vial from the sample chamber empty the vial rinse the vial and c...

Страница 69: ... an error message appears please repeat adjustment Notes 1 The same batch of SPADNS reagent solution must be used for adjustment and test The adjustment process needs to be performed for each new batch of SPADNS reagent solution see Standard methods 20th 1998 APHA AWWA WEF 4500 F D S 4 82 2 As the test result is highly the dependent on the exact sample and reagent volumes the sample and reagent vo...

Страница 70: ...ion If the user calibration has deleted the factory calibration is automatically activated Remarks The fluoride method cannot be calibrated with mode 45 since the test requires a calibration related to the batch of liquid reagent SPADNS mode 40 chapter Fluoride Method 170 see above No Method Recommended range for user calibration 35 Alkalinity p 100 300 ml l CaCO3 30 Alkalinity total 50 150 mg l C...

Страница 71: ...0 Nitrate LR 0 5 0 7 mg l N 270 Nitrate 0 2 0 3 mg l N 290 Oxygen active Calibration with basic test 100 chlorine free 300 Ozone DPD Calibration with basic test 100 chlorine free 320 Phosphate LR 1 3mg l PO4 321 Phosphate HR 30 50 mg l PO4 329 pH value LR 6 0 6 6 330 pH value 7 6 8 0 332 pH value HR 8 6 9 0 340 Potassium 3 mg l K 350 Silica 0 5 1 5 mg l SiO2 212 Sodium hypochlorite 8 355 Sulfate 5...

Страница 72: ...corresponds to the value of the standard Confirm with key Cancel user calibration by pressing ESC key The display shows Now the method name is displayed inverse and the test result is calculated with new calibration factor Delete User Calibration This chapter only applies for the methods which can be user calibrated Select the required method 100 Chlorine T 0 02 6 mg l Cl2 0 90 mg l free Cl2 User ...

Страница 73: ...the methods a Method b Range c Date and time d Differentiation of results e Detailed operator instruction f Compliance with reaction periods If the Profi Mode is active the photometer provides only a minimum of operator instructions The criteria specified above in d e and f are no longer included Press MODE Shift 5 0 keys Confirm with key The display shows Press Shift 0 keys to switch the Profi Mo...

Страница 74: ...the instrument is first being used for an OTZ compatible method and One Time Zero is activated the instrument will request a new Zero with prepare OT Zero Perform Zero as described in the method This Zero will be stored and used for all methods with OTZ function until the instrument is switched off If necessary a new Zero can be performed by pressing ZERO key at any time Press MODE Shift 5 5 keys ...

Страница 75: ...thod list is displayed Method with a point behind the method number will be displayed in the method selection list Methods without a point will not be displayed in the method selection list Press arrow key or to select the required method from the displayed list Switch with F2 key between active and inactive Select next method activate or inactivate as required and repeat Confirm with key Cancel w...

Страница 76: ...ss Shift 1 keys to display only one method in the method selection list Press Shift 0 keys to keep the valid method selection The instrument goes back to mode menu automatically User Concentration Methods It is possible to enter and store up to 10 User Concentration Methods Therefore you need 2 to 14 standards of know concentration and one blank deionised water or reagent blank value The standards...

Страница 77: ...ws the query Or Press Shift 0 or ESC keys to go back to method no query Press Shift 1 keys to start entry mode The display shows Enter the required wavelength e g Shift 2 for 560 nm The display shows Press arrow key or to select the required unit Confirm with key User concentr choose no _ _ _ 850 859 User concentr Overwrite conc meth YES 1 NO 0 User concentr wavelength 1 530 nm 4 430 nm 2 560 nm 5...

Страница 78: ...TANDARDS OF KNOWN CONCENTRATION The display shows Prepare Zero and press ZERO key Note Use deionised water or reagent blank value The display shows Enter the concentration of the first standard e g Shift 0 0 5 Press ESC to delete a digit Press F1 key to reset numerical input Confirm with key The display shows User concentr choose resolution 1 1 2 0 1 3 0 01 4 0 001 User concentr prepare Zero press...

Страница 79: ...ximum number of measured standards is 14 S1 to S14 If all required standards or the maximum value of 14 standards are measured press Store key The display shows The instrument goes back to the mode menu automatically Now the concentration is stored in the instrument and can be recalled by entering its method number or selecting it from the displayed method list TIP Save all your concentration data...

Страница 80: ...13 567E 02 Press MODE Shift 6 5 keys Confirm with key The display shows Enter a method number in the range from 800 to 824 e g Shift 8 0 0 Confirm with key Note If the entered number has already been used to save a concentration the display shows the query Or Press Shift 0 or ESC keys to go back to method no query Press Shift 1 keys to start entry mode The display shows Enter the required waveleng...

Страница 81: ...ient the input of the exponent is omitted automatically Confirm every input with key The display shows Enter measurement ranges from 2600 mAbs to 2600 mAbs Press arrow key or to change between plus and minus sign Enter the values in Absorbance mAbs for the upper limit Max and the lower limit Min Confirm with key The display shows Press arrow key or to change between plus and minus sign Confirm wit...

Страница 82: ... of power outage e g changing the battery all polynomial data will be lost and must be entered again You might want to use Mode 67 to transfer all polynomial data to a PC Delete User Methods Polynomial or Concentration In principle a valid user method can be overwritten An existing user method polynomial or concentration can be totally deleted as well and is removed out of the method selection lis...

Страница 83: ... stored user methods The display shows e g After data transfer the photometer goes back to mode menu automatically Initialise User Method System Polynomials Concentration Power loss will cause incoherent data The user method system must be initialised with this mode function to set it to a predefined state ATTENTION All stored user methods polynomial concentration are deleted with initialisation P...

Страница 84: ...rm with key If F is selected enter the temperature value in the range between 37 and 128 F The display shows Enter the value for calcium hardness CH in the range between 50 and 1000 mg l CaCO3 and confirm with key The display shows Enter the value for total alkalinity TA in the range between 5 and 800 mg l CaCO3 and confirm with key The display shows Enter the value for total dissolved solids TDS ...

Страница 85: ...e defined range Selection of Temperature Unit Entering the temperature value is possible in degrees Celsius or degrees Fahrenheit Therefore the following preselection is once required Press MODE Shift 7 1 keys Confirm with key The display shows Press Shift 1 keys to select degrees Celsius Press Shift 2 keys to select degrees Fahrenheit The instrument goes back to mode menu automatically Langelier ...

Страница 86: ... to decrease the contrast of the LCD display by 10 units Confirm with key Adjusting Display Contrast Press MODE Shift 8 1 keys Confirm with key The display shows Press arrow key to increase the contrast of the LCD display by one unit Press arrow key to decrease the contrast of the LCD display by one unit Press STORE key to increase contrast of the LCD display by 10 units Press TEST key to decrease...

Страница 87: ...a or to transmit to a PC the optional Trace2o PC Link infra red module is required 2 5 1 Data Printing Besides the PC Link module the following printer is required to print data directly using the USB Interface of the module HP Deskjet 6940 2 5 2 Data Transfer to a Personal Computer Besides the PC Link a transfer programme is required to transmit test results Please find detailed information in th...

Страница 88: ...t in a transparent measurement chamber Wavelength ranges λ1 530 nm IF Δ λ 5 nm λ2 560 nm IF Δ λ 5 nm λ3 610 nm IF Δ λ 6 nm λ4 430 nm IF Δ λ 5 nm λ5 580 nm IF Δ λ 5 nm λ6 660 nm IF Δ λ 5 nm IF Interference filter Wavelength Accuracy 1 nm Photometric Accuracy 2 FS T 20 C 25 C Photometric Resolution 0 005 A Measuring Range of Absorbance 2600 2600 mAbs Protection Conforming to IP 68 1 h 0 1 m Operatio...

Страница 89: ...FICATION To ensure maximum accuracy of test results always use the reagent systems supplied by the instrument manufacturer 3 3 Abbreviations Abbreviation Definition C Degree Celsius centigrade F Degree Fahrenheit F C x 1 8 32 dH Degree German Hardness fH Degree French Hardness eH Degree English Hardness aH Degree American Hardness Abs Absorption unit Extinction E 1000 mAbs 1 Abs 1 A 1 E µg l ppb M...

Страница 90: ...r reagent PP Powder Pack T Tablet TT Tube Test DEHA N N Diethylhydroxylamine DPD Diethyl p phenylenediamine DTNB Ellman s reagent PAN 1 2 Pyridylazo 2 napthol PMAB Paradimethylaminobenzaldehyde PPST 3 2 Pyridyl 5 6 bis 4 phenylsulfonic acid 1 2 4 triazine TPTZ 2 4 6 Tri 92 Pyridyl 1 3 5 triazine ...

Страница 91: ...Storage system error Use mode 34 Mains power fails or is not connected Insert or change battery Delete data with mode 34 Battery warning Warning signal every 3 minutes Capacity of the battery is too low change the batteries Warning signal every 12 seconds Warning signal the instrument switches itself off Change the batteries Jus Overrange E4 The user calibration is out of the accepted range Please...

Страница 92: ...Example 1 Example 1 The readings for free and total chlorine are different but considering the tolerances of each reading they are the same For this reason the combined chlorine is most likely zero Example 2 Example 2 The reading for free chlorine is under the detection limit The instrument is not able to calculate the combined chlorine In this case the combined chlorine is most likely the same as...

Страница 93: ...on between differentiated free or total Profi Mode is switched on Switch Profi Mode off with mode 50 The pre programmed countdown is not displayed Countdown is not activated and or the Profi Mode is activated Switch the countdown on with mode 13 and or switch the Profi Mode off with mode 50 It seems that a method is not available Method is not activated in the user method list Activate the require...

Страница 94: ...Trace2o Ltd Technology Centre Wagtech Court Station Road Thatcham Berkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales trace2o com www trace2o com ...

Страница 95: ...No 1 tablet HT10 Ammonia No 2 tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started Switch the unit on using the power key Select the Ammonia method by depressing the cursor keys until Ammonia T is displayed or use the shortcut by pressing holding shift followed by 60 Release s...

Страница 96: ...ces are visible Add one HT10 Ammonia No 2 tablet straight from the foil to the same water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press the Test key Wait...

Страница 97: ...le The tablets must be added in the correct sequence Ammonia No 1 tablet will only dissolve fully after Ammonia No 2 tablet is added The tablets should be added direct from the foil avoiding contact with hands or surfaces The temperature is critical for full sample development Below 20 C increase reaction time to 15 minutes The tablets are unsuitable for determination of ammonia in sea water or br...

Страница 98: ... rod HT15 DPD No 1 tablet HT16 DPD No 3 tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started Switch the unit on using the power key Select the Chlorine method by depressing the cursor keys until Chlorine T is displayed or use the shortcut by pressing holding shift followed by ...

Страница 99: ...al are aligned Press the zero key Wait until the following is displayed Sample preparation Free Chlorine Add one HT15 DPD No 1 tablet straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Free Chlorine Place the vial in the sample cha...

Страница 100: ...t the marks on the instrument and vial are aligned Press the Test key Wait for a reaction period of 2 minutes After the reaction period the measurement starts and the result is displayed in mg l total Chlorine Sample preparation Differentiated Determination Add one HT15 DPD No 1 tablet straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are ...

Страница 101: ...Tolerance 0 005mg l Notes For best results rinse vials thoroughly between with deionised water Ensure that the outside of the vials are clean dry and free from fingerprints Always handle vials by the lid where possible For deep cleaning of vials immerse in Sodium Hypochlorite solution for 1 hour and rinse with deionised water The tablets should be added direct from the foil avoiding contact with h...

Страница 102: ...st tablet 10ml vial Stirring rod Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started Switch the unit on using the power key Select the Nitrate method by depressing the cursor keys until Nitrate T is displayed or use the shortcut by pressing holding shift followed b...

Страница 103: ...dd one Nitrate Test tablet straight from the foil to the water sample Close the tube tightly with the cap and swirl vigorously for one minute Stand the tube upright allow contents to settle then gently invert three to four times Allow to stand for a further 2 minutes then open and wipe carefully around the rim to remove solid particles Decant 10ml of the treated solution into the 10ml vial Add one...

Страница 104: ...side of the vials are clean dry and free from fingerprints Always handle vials by the lid where possible The tablets should be added direct from the foil avoiding contact with hands or surfaces Nitrite present in the sample will also react leading to a higher result To correct carry out a nitrite test P270 on the same sample and subtract the result Nitrate concentrations above 1 mg L can be dilute...

Страница 105: ...rod Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started Switch the unit on using the power key Select the Nitrite method by depressing the cursor keys until Nitrite T is displayed or use the shortcut by pressing holding shift followed by 270 Release shift and press...

Страница 106: ...t straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press the Test key Wait for a reaction period of 10 minutes After the react...

Страница 107: ...e of the vials are clean dry and free from fingerprints Always handle vials by the lid where possible The tablets should be added direct from the foil avoiding contact with hands or surfaces Interferences may occur from presence of the following ions Antimony III Iron III Lead Mercury I Silver Chloroplatinate Metavanadate Bismuth Copper II ions may artificially lower test results ...

Страница 108: ...Section 6 HydroCheck HC1000 Multi Parameter Electrochemical Water Tester Instruction Manual ...

Страница 109: ...Temperature Compensation 88 4 7 Manual Temperature Compensation 89 5 1 pH Calibration 89 5 2 Single Point Calibration 89 5 3 Multi Point Calibration 90 5 4 pH Calibration with User Defined Buffers 91 5 5 pH Calibration Report 92 6 1 ORP Calibration 92 6 2 ORP Calibration Report 94 7 1 Conductivity Calibration 94 7 2 Single Point Calibration 96 7 3 Multi Point Calibration 96 7 4 Manual Calibration ...

Страница 110: ...13 1 Appendix 1 How to calculate the temperature coefficient 109 13 2 Appendix 2 How to calculate the TDS conversion factor 109 13 3 Appendix 3 Pressure vs Altitude Table 111 14 1 Warranty 112 ...

Страница 111: ...0 Ambient temperature is greater than 0o C and less than 60o C No potential electromagnetic interference The following list describes the standard accessories of the meter After the unpacking please check all accessories are complete If any are damaged or missing please contact nearest distributor 1 3 List of Accessories pH Electrode Conductivity Electrode Dissolved Oxygen Electrode pH Buffer Solu...

Страница 112: ...rane keypad Words and symbols describe functions of each key The direction keys are multi function left right and up down keys INDEX KEY DESCRIPTION Power the meter ON OFF Holds the current measured value press the key again to resume measuring In the calibration or setting mode returns to measurement mode mode Toggles between available measurement modes pH mV ORP Ion Conductivity TDS Salinity Res...

Страница 113: ...rough menu Press in temperature setting mode to decrease the setting value enter Confirms the calibration setting value or displayed option Press and hold to turn On Off the backlight 2 3 Connectors The Trace2o HydroCheck HC1000 has three connectors for connecting the various types of sensors CONNECTOR FUNCTION Waterproof S7 Connector pH electrode or optional ORP or Ion Selective Electrodes Waterp...

Страница 114: ...r on the pH electrode into corresponding connector on the instrument Once pushed firmly into place twist to ensure water tightness CONDUCTIVITY ELECTRODE Insert the 3 pin connector on the conductivity electrode into the corresponding connector on the instrument ensuring that the lugs are aligned Once pushed firmly into place tighten the waterproof locking collar ...

Страница 115: ...to place tighten the waterproof locking collar Once connected DO NOT pull on the cables 2 5 Inserting Replacing the Batteries The HC1000 is supplied pre installed with the requisite battery The HC1000 requires 1 x 9V PP3 battery To replace the battery follow the below procedure 1 Remove the battery cover from the rear of the meter 2 Disconnect the snap connector and remove the battery ...

Страница 116: ...Electrolyte Solution for DO Probe The Dissolved Oxygen electrode requires an electrolyte solution which must be kept topped up during normal use To refill follow the below procedure 1 Unscrew the membrane cap from the bottom of the dissolved oxygen probe 2 Fill the membrane cap halfway with electrolyte solution 3 Screw the membrane cap onto the probe excess electrolyte will drain out 4 Be sure the...

Страница 117: ...an 3 Immerse the electrode in the sample solution and stir the solution gently 4 Wait for the reading to stabilise and record the value shown on the display 3 3 ORP Measurement The HC1000 has two millivolt measurement modes ABSOLUTE MILLIVOLT MODE Press the MODE key until the display shows the measurement unit mV the meter enters absolute millivolt measurement mode RELATIVE MILLIVOLT MODE Press th...

Страница 118: ... and measurement unit ppt the meter enters seawater measurement mode 3 Select the desired measurement mode Immerse the electrode in the sample solution Stir the solution gently 4 Wait for the reading to stabilise and record the value shown on the display 3 6 Dissolved Oxygen Measurement The HC1000 is suitable for measuring dissolved oxygen in water wastewater brine and other liquids If the intende...

Страница 119: ...old function is enabled the meter will automatically sense a stable endpoint reading and hold The Hold indicator appears on the display If the Auto Hold function is disabled press the ON OFF key the meter will immediately hold currently displayed value Press the ON OFF key again to resume measuring 3 7 Storing and Recalling Data from Memory The HC1000 allows up to 500 data sets to be stored and re...

Страница 120: ...ter shows the date and time of the reading Format mm dd hh mm 4 Press the key again the display shows the stored data 5 After viewing the memory press the ON OFF key to return to measurement mode Enter MR p MR p Page number ...

Страница 121: ...DESCRIPTIO N DEFAU LT Select from common pH buffer standard groups for calibration USA 1 68 4 01 7 00 10 01 12 45 NIST 1 68 4 01 6 96 9 18 12 45 DIN 1 09 4 65 6 79 9 23 12 75 User Defined Buffer Select the number of calibration points to use when calibrating the meter 1 point 2 points 3 points 4 points 5 points Set the resolution for pH measurement 0 001pH 0 01pH 0 1pH Set the temperature unit Deg...

Страница 122: ...he number of calibration points to use when calibrating the meter 1 point 2 points 3 points 4 points 5 points Pure water coefficient will be calculated and applied automatically for ultra pure water measurement if enabled Enable Disable Set the normalization temperature for conductivity measurement and calibration 25o C 20o C Set the default TDS conversion factor Setting Range 0 40 to 1 00 0 5 Set...

Страница 123: ...heric pressure coefficient Setting Range 450 to 850mmHg 760 Select the salinity coefficient of sample solution Setting Range 0 0 to 50 0ppt 0 0 Set the resolution for DO measurement 0 01mg L 0 1 0 1mg L 1 Set the default measurement units Degrees Celsius Degrees Fahrenheit mg L Milligrams per litre ppm Parts per million mmHg Pressure unit kPa Pressure unit ...

Страница 124: ...ze slowly but improves accuracy Low High When the auto hold function is enabled the meter will automatically sense a stable end point reading and hold Enable Disable When the auto off power is enabled the meter will turn off automatically if there are no key presses within the specified time period 10 minutes 20 minutes 30 minutes Disable When the calibration due reminder is enabled if you do not ...

Страница 125: ...ets the date and time of the meter Clear all stored data Enable Disable Reset function allows user to restore the meter to factory default settings When this function is used all calibration values and settings will be lost or reset Enable Disable ...

Страница 126: ...display shows the currently selected option 5 Press or key to select the desired option 6 Press ENTER key to confirm the meter returns to measurement mode Setting is completed 4 3 Exiting setup mode During the setup mode if you want to exit setup menu press MEAS key the meter will return to measurement mode immediately 4 4 Setting the Date and Time The HC1000 has a real time clock that is used to ...

Страница 127: ...n finished press ENTER key to confirm and the meter returns to measurement mode 4 5 Temperature Compensation For accurate results either manual or automatic temperature compensation must be enabled before measurement or calibration 4 6 Automatic Temperature Compensation Insert the 6 pin connector on the temperature probe into the corresponding connector on the instrument ensuring that the lugs are...

Страница 128: ...tandard Buffer Options pH 1 68 4 01 6 86 9 18 12 45 DIN Standard Buffer Options pH 1 09 4 65 6 79 9 23 12 75 If you selected the user defined option in the setup menu the meter will allow only 2 point calibration Single point calibration should only be carried out with buffers of pH 7 00 6 86 or 6 79 otherwise calibration will not be accepted Ensure that the meter is calibrated when attaching a ne...

Страница 129: ...isplay will show CAL2 The meter prompts to continue with second calibration point 5 3 3 Rinse the pH electrode with distilled water Dip the electrode into the next buffer solution e g pH4 01 The meter automatically senses the current calibration solution and begins calibrating Calibration indicator begins flashing 5 3 4 Wait for the measured value to stabilise the display shows CAL3 The meter prom...

Страница 130: ...ontinue with second calibration point 5 4 5 Rinse the pH electrode with distilled water Immerse the electrode in the next custom buffer solution 5 4 6 Press or key to increase or decrease displayed value according to the value of the custom buffer and press ENTER key to confirm Calibration indicator begins flashing 5 4 7 Wait for the measured value to stabilise the display shows END Once calibrati...

Страница 131: ...he meter shows OFS zero point offset potential 5 5 5 Press key again the meter shows the slope of each pH buffer group 5 5 6 Press the ON OFF key to return to measurement mode If the meter is not calibrated or calibration is not successful the display will show only 6 1 ORP Calibration The HC1000 allows single point calibration in the relative mV mode but calibration is not necessary unless and ex...

Страница 132: ... 3 Press CAL key the meter shows the current measured value 4 Press or key to set the displayed value press ENTER key to confirm The Calibration indicator begins flashing 5 Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to measurement mode automatically Enter ...

Страница 133: ... the setup menu If the current option is USER the automatic calibration function will be disabled Refer to Setup Menu section In the automatic calibration mode the meter allows up to a 5 point calibration To ensure higher accuracy at least a 3 point calibration is recommended Alternatively it is advised to select a standard value close to the sample value under measurement The meter will automatic...

Страница 134: ...200 2000µS cm 700 1700µS cm 1413µS cm 2 20mS cm 7 17mS cm 12 88mS cm 20 200mS cm 70 170mS cm 111 8mS cm ...

Страница 135: ...hows the detected calibration standard e g 1413µS cm 7 2 5 Press ENTER key to confirm Calibration indicator begins flashing 7 2 6 Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to measurement mode automatically 7 3 Multi Point Calibration 7 3 1 Make sure that you have selected 2 to 5 points calibration in setup menu 7 3 2 Repeat steps ...

Страница 136: ...n standard e g 12 88mS cm 7 3 4 Press ENTER key to confirm Calibration indicator begins flashing 7 3 5 Wait for the measured value to stabilise the display shows CAL3 The meter prompts you to continue with third point calibration 7 3 6 Repeat steps 2 3 to 2 4 above until the display shows END Once calibration is complete the meter returns to measurement mode automatically Enter ...

Страница 137: ...or 3 seconds to enter setup menu the display shows CELL option 7 4 3 Press ENTER key to confirm the meter enters the cell constant setting mode 7 4 4 Press or key to select USER option 7 4 5 Press ENTER key to confirm the meter enters User Defined mode 7 4 6 Press or key to set each digit according to the value marked on the electrode 7 4 7 Press ENTER key to confirm until the setting values stop ...

Страница 138: ...ows calibration point and calibration factor e g K 0 998 5 After the browsing press MEAS key to exit the current mode EXIT THE CALIBRATION To exit calibration press the ON OFF key at any point the meter will return to measurement mode immediately 8 1 DO Calibration in Saturation Mode The HC1000 dissolved oxygen probe can be calibrated quickly in air In the percentage saturation mode the meter is a...

Страница 139: ...L key the meter enters calibration mode the display shows 100 CAL1 8 2 4 Hold the dissolved oxygen probe in the air press ENTER key to confirm Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to measurement mode automatically ...

Страница 140: ...Wait for the measured value to stabilise the display shows 100 CAL2 The meter prompts you to continue with second point calibration 8 3 7 Immerse the dissolved oxygen probe in air saturated water for 3 to 5 minutes press ENTER key to confirm Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to measurement mode automatically N B Performing...

Страница 141: ......

Страница 142: ...eric pressure coefficient in the setup menu Refer to Setup Menu section 9 1 4 Press CAL key the display shows 8 25mg L CAL1 25o C 9 1 5 Dip the dissolved oxygen probe into the air saturated water for 3 to 5 minutes 9 1 6 Press ENTER key to confirm Calibration indicator begins flashing 9 1 7 Wait for the measured value to stabilise the display shows END Once calibration is complete the meter return...

Страница 143: ...libration indicator begins flashing 9 2 6 Wait for the measured value to stabilise the display shows 8 25 CAL2 The meter prompts you to continue with second point calibration 9 2 7 Immerse the dissolved oxygen probe in air saturated water for 3 to 5 minutes press ENTER key to confirm Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to me...

Страница 144: ... the temperature reading displayed differs from that of an accurate thermometer 10 1 1 Press and hold the MODE key for 3 seconds to enter temperature calibration mode the display shows current temperature reading 10 1 2 Press or key to set the temperature value 10 1 3 Press ENTER key to confirm Calibration is completed ...

Страница 145: ...TRODE If stored and cleaned properly the electrode should be ready for immediate use However a dehydrated bulb may cause sluggish response To rehydrate the bulb immerse the electrode in a pH 4 01 buffer solution for 10 to 30 minutes If this fails the electrode requires activation Soak the pH electrode in 0 1M HCl for 5 minutes Remove and rinse with deionised water then place in 0 1M NaOH for 5 min...

Страница 146: ...ds unscrew membrane cap and rinse the probe s cathode anode and the membrane with deionised water then soak up residual water with filter paper and re install the probe 12 1Troubleshooting LCD DISPLAY CAUSE CORRECTIVE ACTION Electrode dried out Soak the pH electrode in 3M KCL solution for 15 minutes Soak the conductivity electrode in tap water for a few minutes Measured value is out of range Check...

Страница 147: ...Zero oxygen solution is contaminated Replace the calibration solution ...

Страница 148: ...alue TA and conductivity value CTA 5 Repeat the steps above whilst dipping the electrode and probe into same sample solution B Make sure both sample solutions have about 5o C to 10o C difference 6 Record the temperature value TB and conductivity value CTB 7 Calculate the temperature coefficient of sample solution according to the formula shown above 8 Enter the calculated temperature coefficient i...

Страница 149: ...l Conductivity the meter measured conductivity value For example Dissolve 64 grams of potassium chloride reagent in 1L distilled water If its conductivity value is 100mS cm then TDS conversion factor is 0 64 ...

Страница 150: ...60 100 100 1 750 200 98 8 741 300 97 6 732 400 96 4 723 500 95 2 714 600 94 0 705 700 92 8 696 800 91 7 688 900 90 5 679 1000 89 4 671 1100 88 3 662 1200 87 2 654 1300 86 1 646 1400 85 0 638 1500 84 0 630 1600 82 9 622 1700 81 9 614 1800 80 9 607 1900 79 9 599 2000 78 9 592 ...

Страница 151: ...ed by the Trace2o Ltd standard warranty terms and conditions available via email or via download from www trace2o com In the event that any technical assistance is required Trace2o Customer service department will be happy to assist Contact details as follows Trace2o Ltd The Technology Centre Station Road Thatcham Berkshire RG19 4HZ UK T 01635 866772 E Technical Trace2o com ...

Страница 152: ...NOTES ...

Страница 153: ...Trace2o Limited The Technology Centre Wagtech Court Station Road Thatcham Berkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales Trace2o com www Trace2o com ...

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