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Operator’s Manual

Rev 9/20/18

pg. 45

TDF Analysis (AOAC 985.29 / 2001.03)

A TDF analysis directly measures the amount of TDF within a given sample without separately measuring the IDF and SDF
fractions. TDF can be determined using multiple methods. This section describes the procedure for using the ANKOM

TDF

Dietary Fiber Analyzer to determine TDF based on the AOAC 985.29 and 2001.03 methods.

This analysis requires an SDF filter bag (ANKOM DF-S) for the precipitation process and a non-filter IDF

Flow-Thru

bag

(ANKOM DF-FT) for the digestion process. When starting a new run, the ANKOM

TDF

Dietary Fiber Analyzer must have bags

installed at all stations (a total of twelve bags).

To enhance the productivity of your instrument, you can begin the IDF process of a
new run while the SDF process of a previous run is finishing. See the “Productivity
Enhancement” section of this manual for more details.

To perform a TDF analysis, follow the steps below.

1.

Prepare chemicals and enzymes.

When using the ANKOM

TDF

Dietary Fiber Analyzer for the AOAC 985.29 or 2001.03 methods use the chemicals and

enzymes referred to therein. See Appendix A of this manual for the list of chemicals and enzymes and the instructions for
how to prepare them for use in this instrument.

2.

De-fat your samples as needed according to the official methods. See "Appendix C

–

De-fatting

Procedure" in this manual for an ANKOM procedure.

3.

Label the bags using a Solvent Resistant Marker.

4.

Prepare for data collection.

You will need a place to store the data collected during this analysis. For your convenience a CD titled “Calculation
Templates” is included with the instrument. This CD has TDF Data Spreadsheets that can be used for this analysis. Please
read the “Instructions” tab in the MS-Excel file for information about the spreadsheets.

5.

Weigh filter bags.

Using a Bag Weigh Holder is critical to eliminate the effects of static electricity
during the weighing process.

Roll or fold each bag and place it in a tared Bag Weigh Holder. Place the Bag Weigh Holder in the center of a balance
and record the weight.

Because different balances have different sensitivities, the Bag Weigh Holder
should be placed in the center of the balance for best results.

SDF Bag (longer bag with filter)

IDF

Flow-thru

Bag (shorter bag / no filter)

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Содержание HS

Страница 1: ...Rev 9 20 18 Operator s Manual 2052 O Neil Rd Macedon NY 14502 Telephone 315 986 8090 Fax 315 986 8091 www ankom com ...

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Страница 3: ...NKOMTDF Dietary Fiber Analyzer 16 Blanks 16 IDF SDF Analysis 17 TDF Analysis AOAC 991 43 31 TDF Analysis AOAC 985 29 2001 03 44 Protein Determination 59 Ash Determination 61 Productivity Enhancement 63 Status Screen 67 Fault Handling 71 Diagnostics Mode 73 Periodic Maintenance 81 Troubleshooting Replacement Parts 82 QC Calibration Checks 83 Appendix A Reagents AOAC 991 43 985 29 2001 03 89 Appendi...

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Страница 5: ...l as instrument control helping you to achieve the best possible results Please review the entire manual before you begin operating this product Warranty ANKOM Technology warrants the ANKOMTDF Dietary Fiber Analyzer against any defects in workmanship or material for one year after the original date of purchase This warranty does not include damage to the instrument resulting from neglect or misuse...

Страница 6: ...te results using weights and corrections AOAC 991 43 Method IDF SDF assay with ANKOMTDF Dietary Fiber Analyzer automation Below is the AOAC 991 43 method flow when using the ANKOMTDF Dietary Fiber Analyzer Steps highlighted in YELLOW represent tasks done by a technician Steps highlighted in GREEN 1a 1g 9 19 and 30 are no longer needed because of Filter Bag Technology All other steps including mixi...

Страница 7: ... bags when configured to recover TDF residue The automated fiber recovery is achieved by first digesting samples within bags using enzyme treatments Depending on the filter bag configuration the instrument then collects the IDF and SDF residue using two separate filters or collects the TDF residue using one filter Once captured in the filters the residue is dried weighed and corrected for protein ...

Страница 8: ...95 EtOH Container TDF42 HCl Container TDF38 Enzyme Containers TDF35 TDF36 TDF37 Nitrogen Supply Port 80 100 psi 5 5 6 9 bar Front Cover Arm Power Switch Front Cover TDF56 78 EtOH Container TDF41 Buffer Container TDF39 Distilled Water Container TDF40 Front Cover Arm Three Container Holder Single Container Holder Pressure Gauge ...

Страница 9: ...lve Stations 4 5 6 In Line Heater Tubing Support Panel IDF Inlet Valve Stations 1 2 3 SDF Inlet Valve Stations 1 2 3 Waste Valves 1 6 Supply Valves Water Buffer 78 EtOH 95 EtOH Vacuum Sensor Supply Manifold Clamp Bar Cylinder 1 of 8 HCl Supply Valve Peristaltic Pump Pressure Sensors Drain Tube Supply Valves Amylase Protease AMG Torsion Bar for Front Cover ...

Страница 10: ... exceed 100 C 212 F Failure to observe this caution may result in burns Hazardous Voltages Do NOT operate the instrument with the back of the Main Electrical Cabinet removed Hazardous voltages are present during operation Failure to observe this caution may result in electrical shock or electrocution Hazardous Materials Ethanol is used within this instrument Follow both local and federal regulatio...

Страница 11: ...the sleeve of the instrument shipping container Lift the instrument off of the shipping container bottom and place it on a surface that is firm and level in an area near a Nitrogen Supply and a drain or chemical waste container Waste fluids are drained from the instrument using gravity Therefore position the instrument so that the drain tube can run down from the Waste Tray The instrument must not...

Страница 12: ...er assy TDF38 1 Mes Tris Buffer Container assy TDF39 1 Distilled Water Container assy TDF40 1 78 EtOH Container assy TDF41 1 95 EtOH Container assy TDF42 6 Filtrate Cups TDF53 1 Control Panel assy TDF17 1 Bag Weigh Holder assy TDF52 1 Drying Rack assy TDF50 2 Desiccant Pouches X45 1 3 meter Air Tube 8216 1 CD with Calculation Templates 1 three container holder 8191 1 single container holder 8192 1...

Страница 13: ...e filtrate cups in place on the drain tray However the red plug can be pulled from the lower port to drain the filtrate into the drain tray Store the plug in the upper port 5 Connect the Drain Tube to the Waste Tray Connect and secure the Drain Tube to the Waste Tray using the supplied Drain Tube Clamp Run it to either a drain or a waste container avoiding twists in the tube If necessary cut the D...

Страница 14: ...e holder on the right side of the instrument place the Distilled Water Container in the front section the Buffer Container in the middle section and the 78 EtOH Container in the back section The 95 EtOH Container sits on the bench behind the container holder To ensure proper fluid delivery you must use the Chemical Containers provided with the instrument and place them in their specified locations...

Страница 15: ... the Nitrogen Supply If you want to use a separate tube to connect your instrument to a Nitrogen Supply the Nitrogen Supply Port has a Push to Connect fitting that accepts OD tubing Acceptable tubing materials include Nylon Polyurethane Polyolefin and Polytetrafluoroethylene PTFE The tubing material must be suitable for the specified pressure 12 Verify that the High and Low Pressure Regulators are...

Страница 16: ... filter In this analysis the TDF value consists of the weighed residue corrected for protein and ash IDF Flow Thru no filter SDF A computer controlled Diagnostics mode is provided through the Control Panel for maintenance and troubleshooting purposes To maximize productivity this instrument also allows a user to start new procedures IDF SDF and TDF before the previous ones have completed See the P...

Страница 17: ...uctions for how to prepare them for use in this instrument 2 De fat your samples as needed according to the official methods See Appendix C De fatting Procedure in this manual for an ANKOM procedure 3 Label the filter bags using a Solvent Resistant Marker 4 Prepare for data collection You will need a place to store the data collected during this analysis For your convenience a CD titled Calculatio...

Страница 18: ...ons on the Touch Screen Display and buttons below the screen When your instrument is initialized and ready to operate the following screen will be displayed Although the instrument works in accordance with AACC and NMKL methods the Touch Screen Display only refers to the AOAC methods 9 1 If this is the first time the instrument is being operated after being installed or after sitting unused for a ...

Страница 19: ...The following Select a Function screen will be displayed again 9 2 Press the AOAC 991 43 IDF SDF button The following screen will be displayed Notice that the function you selected is now displayed in the top right corner of the screen and a back button is displayed in the top left corner of the screen SDF Bags and clamp bar D installed AOAC 991 43 IDF SDF Select a Function AOAC 991 43 IDF SDF AOA...

Страница 20: ...top part of the bag Pull the bag up so that the top of the bag is about 35 mm 1 375 inches above the top of Clamp Bar C and return the Delivery Nozzle to its original position This will hold the back of the bag in place As part of normal operation solution from the IDF bag will flow into the SDF bag Therefore when installing the SDF filter bags it is very important to position them high enough ver...

Страница 21: ...on the Touch Screen Display to pinch the bags just above the filter The following screen will be displayed 11 Add DE to the SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer 11 1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle When adding DE to the filter bags it is very important to...

Страница 22: ...ozzle so that the Delivery Nozzle is inside the top part of the bag Pull the bag up so that the top of the filter part of the IDF bag is just below the bottom of Clamp Bar B and return the Delivery Nozzle to its original position This will hold the back of the bag in place 12 3 With the bag held by the Delivery Nozzle center it horizontally between the Centering Lines on the back part of Clamp Bar...

Страница 23: ... on the Touch Screen Display to pinch the IDF bags just above the filter The mixing pads will make contact with the bags when Clamp Bar B pinches the IDF bags The following screen will be displayed 13 Add samples to the IDF bags 13 1 Open the top of the IDF bag and transfer the sample from a weigh tin into the IDF bag by folding the tin and then dipping it down into the bag below the tip of the De...

Страница 24: ...tter on the top of the bar and the rubber material facing in toward the instrument Press the check mark button on the Touch Screen Display The following screen will be displayed 14 Hook the front of each SDF bag in place 14 1 Secure the SDF filter bags in place with the hooks located on the front part of Clamp Bar C Hooking the bags in place will put a physical hole in the front of each SDF bag 14...

Страница 25: ... fluid levels above the Minimum Level lines on the chemical containers and at least 15 ml of each enzyme Add fluids and enzymes as necessary Do NOT leave the enzyme ports on the instrument open to the air or the enzymes in the valves may dry up and plug the ports With all fluid containers filled to the proper levels press the check mark button on the Touch Screen Display The following screen will ...

Страница 26: ...m again The initial filter times shown when you first run the instrument are based on factory experience During filtration the computer allows you to bypass the filter time if you notice the filtering is complete The computer also allows you to add time during filtering if needed see the Status Screen section of this manual for more detail When all of the times shown on the screen are what you wan...

Страница 27: ...owing screen will be displayed If the fluid lines are already charged properly or when the line charge is complete the following screen will be displayed When the tube integrity check is complete the instrument will automatically execute the IDF SDF procedure stopping only for faults aborts and manual pH measurement if enabled The Status screen will show actions and faults as they occur during the...

Страница 28: ...he IDF residue that has been collected in the IDF bag must be manually rinsed twice with acetone It is recommended that you use an ANKOM TDF51 Rinse Stand sold separately for rinsing filter bags with acetone To rinse the IDF filter bags with acetone using the ANKOM TDF51 Rinse Stand follow the steps below 22 1 Remove the IDF bags from the instrument 22 2 Place the bags on the Rinse Stand by slidin...

Страница 29: ...one to evaporate from the bags Hazardous Materials Do NOT heat seal or place bags in an oven until all acetone has evaporated 23 6 Seal each bag just above the filter to keep all residue contained to the filter area while handling the bags Sealing each bag as close to the filter as possible allows you to trim the maximum amount of polypropylene from the bag before determining the protein content 2...

Страница 30: ...2 Residue for duplicate blanks g fBF Final Blank Filter Bag g fBS Initial Blank Filter Bag g PB Protein of Blank Filter Bag g AB Ash of Blank Filter Bag g 30 Calculate the SDF value SDF R1 R2 2 P A B M1 M2 2 fF1 fS1 D1 fF2 fS2 D2 2 P1 A2 D2 B M1 M2 2 Where M1 M2 Original wt of duplicate samples adjusted for pre treatment fat and sugar losses g R1 R2 Residue for duplicate samples g fF Final Filter ...

Страница 31: ...MKL 129 2003 methods use the chemicals and enzymes referred to therein See Appendix A of this manual for the list of chemicals and enzymes and the instructions for how to prepare them for use in this instrument 2 De fat your samples as needed according to the official methods See Appendix C De fatting Procedure in this manual for an ANKOM procedure 3 Label the bags using a Solvent Resistant Marker...

Страница 32: ...ons on the Touch Screen Display and buttons below the screen When your instrument is initialized and ready to operate the following screen will be displayed Although the instrument works in accordance with AACC and NMKL methods the Touch Screen Display only refers to the AOAC methods 9 1 If this is the first time the instrument is being operated after being installed or after sitting unused for a ...

Страница 33: ...ice The following Select a Function screen will be displayed again 9 2 Press the AOAC 991 43 TDF button The following screen will be displayed Notice that the function you selected is now displayed in the top right corner of the screen and a back button is displayed in the top left corner of the screen SDF Bags and clamp bar D installed AOAC 991 43 TDF Select a Function AOAC 991 43 IDF SDF AOAC 98...

Страница 34: ... of the bag Pull the bag up so that the top of the bag is about 35 mm 1 375 inches above the top of Clamp Bar C and return the Delivery Nozzle to its original position This will hold the back of the bag in place As part of normal operation solution from the IDF Flow thru bag will flow into the SDF bag Therefore when installing the SDF filter bags it is very important to position them high enough v...

Страница 35: ... mark button on the Touch Screen Display to pinch the bags The following screen will be displayed 11 Add DE to the SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer 11 1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle When adding DE to the filter bags it is very important to keep it ...

Страница 36: ...e so that the Delivery Nozzle is inside the top part of the bag Pull the bag up so that the top of the bag is about 35 mm 1 375 inches above the top of Clamp Bar A and return the Delivery Nozzle to its original position This will hold the back of the bag in place 12 3 With the bag held by the Delivery Nozzle center it horizontally between the Centering Lines on the back part of Clamp Bar A For pro...

Страница 37: ...ouch Screen Display to pinch the IDF Flow Thru bags The mixing pads will make contact with the bags when Clamp Bar B pinches the IDF Flow Thru bags The following screen will be displayed 13 Add samples to the IDF Flow thru bags 13 1 Open the top of the IDF Flow thru bag and transfer the sample from a weigh tin into the bag by folding the tin and then dipping it down into the bag below the tip of t...

Страница 38: ...r material facing in toward the instrument Press the check mark button on the Touch Screen Display The following screen will be displayed 14 Hook the front of each SDF bag in place 14 1 Secure the SDF filter bags in place with the hooks located on the front part of Clamp Bar C Hooking the bags in place will put a physical hole in the front of each SDF bag 14 2 Press the check mark button on the To...

Страница 39: ...with fluid levels above the Minimum Level lines on the chemical containers and at least 15 ml of each enzyme Add fluids and enzymes as necessary Do NOT leave the enzyme ports on the instrument open to the air or the enzymes in the valves may dry up and plug the ports With all fluid containers filled to the proper levels press the check mark button on the Touch Screen Display The following screen w...

Страница 40: ...nter will remain until you change them again The initial filter times shown when you first run the instrument are based on factory experience During filtration the computer allows you to bypass the filter time if you notice the filtering is complete The computer also allows you to add time during filtering if needed see the Status Screen section of this manual for more detail When all of the times...

Страница 41: ...llowing screen will be displayed If the fluid lines are already charged properly or when the line charge is complete the following screen will be displayed When the tube integrity check is complete the instrument will automatically execute the TDF procedure stopping only for faults aborts and manual pH measurement if enabled The Status screen will show actions and faults as they occur during the a...

Страница 42: ...mplete If you notice sample sticking to an IDF Flow thru bag use 78 EtOH in a wash bottle to rinse as needed 23 Rinse the SDF filter bags with acetone After the instrument has completed a TDF process the TDF residue that has been collected in the SDF bag must be manually rinsed with acetone It is recommended that you use an ANKOM TDF51 Rinse Stand sold separately for rinsing filter bags with aceto...

Страница 43: ...er of a balance 25 3 Record the weight of the filter bag 25 4 Repeat steps 25 1 25 3 for each SDF filter bag used in the fiber analysis 26 Determine the Protein content within the TDF residue See the Protein Determination section of this manual for recommended procedures 27 Determine the Ash content within the TDF residue See the Ash Determination section of this manual for recommended procedures ...

Страница 44: ...Operator s Manual pg 44 Rev 9 20 18 This page intentionally left blank ...

Страница 45: ... or 2001 03 methods use the chemicals and enzymes referred to therein See Appendix A of this manual for the list of chemicals and enzymes and the instructions for how to prepare them for use in this instrument 2 De fat your samples as needed according to the official methods See Appendix C De fatting Procedure in this manual for an ANKOM procedure 3 Label the bags using a Solvent Resistant Marker ...

Страница 46: ...ons on the Touch Screen Display and buttons below the screen When your instrument is initialized and ready to operate the following screen will be displayed Although the instrument works in accordance with AACC and NMKL methods the Touch Screen Display only refers to the AOAC methods 9 1 If this is the first time the instrument is being operated after being installed or after sitting unused for a ...

Страница 47: ... 2 Press either the AOAC 985 29 TDF button or the AOAC 2001 03 TDF button depending on the method you re doing One of the following screens will be displayed Notice that the function you selected is now displayed in the top right corner of the screen and a back button is displayed in the top left corner of the screen SDF Bags and clamp bar D installed AOAC 2001 03 TDF SDF Bags and clamp bar D inst...

Страница 48: ... of the bag Pull the bag up so that the top of the bag is about 35 mm 1 375 inches above the top of Clamp Bar C and return the Delivery Nozzle to its original position This will hold the back of the bag in place As part of normal operation solution from the IDF Flow thru bag will flow into the SDF bag Therefore when installing the SDF filter bags it is very important to position them high enough v...

Страница 49: ...y to pinch the bags One of the following screens will be displayed 11 Add DE to the SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer 11 1 Open the top of the SDF bag and add a weigh tin of DE to the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle When adding DE to the filter bags it is very important to keep it below the tip of the Delivery N...

Страница 50: ...le is inside the top part of the bag Pull the bag up so that the top of the bag is about 35 mm 1 375 inches above the top of Clamp Bar A and return the Delivery Nozzle to its original position This will hold the back of the bag in place 12 3 With the bag held by the Delivery Nozzle center it horizontally between the Centering Lines on the back part of Clamp Bar A For proper mixing during the IDF p...

Страница 51: ...h the IDF Flow Thru bags The mixing pads will make contact with the bags when Clamp Bar B pinches the IDF Flow Thru bags One of the following screens will be displayed 13 Add samples to the IDF Flow thru bags 13 1 Open the top of the IDF Flow thru bag and transfer the sample from a weigh tin into the bag by folding the tin and then dipping it down into the bag below the tip of the Delivery Nozzle ...

Страница 52: ...button on the Touch Screen Display One of the following screens will be displayed 14 Hook the front of each SDF bag in place 14 1 Secure the SDF filter bags in place with the hooks located on the front part of Clamp Bar C Hooking the bags in place will put a physical hole in the front of each SDF bag 14 2 Press the check mark button on the Touch Screen Display If you ran a method other than 985 29...

Страница 53: ...t 15 ml of each enzyme Add fluids and enzymes as necessary Do NOT leave the enzyme ports on the instrument open to the air or the enzymes in the valves may dry up and plug the ports With all fluid containers filled to the proper levels press the check mark button on the Touch Screen Display One of the following screens will be displayed AOAC 2001 03 TDF Nitrogen supply ON AOAC 985 29 TDF Nitrogen ...

Страница 54: ...filter time if you notice the filtering is complete The computer also allows you to add time during filtering if needed see the Status Screen section of this manual for more detail When all of the times shown on the screen are what you want press the check mark button on the Touch Screen Display One of the following screens will be displayed 19 Set the manual pH check for after the Protease digest...

Страница 55: ...ill be displayed If the fluid lines are already charged properly or when the line charge is complete the following screen will be displayed When the tube integrity check is complete the instrument will automatically execute the TDF procedure stopping only for faults aborts and manual pH adjustment The Status screen will show actions and faults as they occur during the automatic operation Testing p...

Страница 56: ... process press the Continue button on the Status screen You will see COMPLETE next to pH required in the upper left corner of the screen 23 Make sure that each sample is completely rinsed down into the SDF filter bags as needed Since the IDF Flow thru bags are not weighed before and after the process it is important to make sure that each sample gets rinsed down into the SDF filter bag when the di...

Страница 57: ... oven until all acetone has evaporated 24 6 With your Heat Sealer set between 3 and 4 settings may vary depending on the heat sealer and the power source press the Heat Sealer arm down Hold the arm down for 3 to 4 seconds after the light goes out to seal each bag just above the filter This keeps all residue contained to the filter area while handling the bags 24 7 Place each bag in the Drying Rack...

Страница 58: ...eatment fat and sugar losses g R1 R2 Residue for duplicate samples g fF Final Filter Bag with residue g fS Initial Filter Bag g D Original wt of Diatomaceous Earth g P Protein of residue and bag g A Ash of residue and bag g B Blank g BR1 BR2 2 PB AB DB fBF1 fBS1 DB1 fBF2 fBS2 DB2 2 PB1 AB2 DB2 BR1 BR2 Residue for duplicate blanks g fBF Final Blank Filter Bag g fBS Initial Blank Filter Bag g PB Pro...

Страница 59: ...ilter bag in thirds from side to side then fold from top to bottom 4 With your Heat Sealer set to 6 setting may vary depending on heat sealer and power source place the exposed polypropylene material from your final fold down and press the Heat Sealer arm down for 3 to 4 seconds to seal each bag Repeat if necessary 5 Label the folded sealed filter bag 6 Repeat steps 1 5 for all IDF bags that will ...

Страница 60: ...ylene edge of the filter bag Repeat if necessary 4 Label the folded sealed filter 5 Repeat steps 1 4 for all SDF bags that will be used for determining protein content 6 Run a Kjeldahl procedure on each filter bag using 27 ml of H2SO4 10 g of K2SO4 and 300 mg of CuSO4 5H2O in a system with 250 ml tubes that have a 42 mm diameter Pellets can be used if an equivalent amount of K2SO4 is added To slow...

Страница 61: ...hen fold the bag in thirds from side to side 2 With your Heat Sealer set to 6 setting may vary depending on heat sealer and power source press the Heat Sealer arm down for 3 to 4 seconds to seal the clear polypropylene part of the bag just above the white filter material 3 Label the folded sealed filter bag and place it in a labeled ashing crucible 4 Repeat steps 1 3 for all IDF bags to be ashed 5...

Страница 62: ...eaving at least 10 mm at the top of the clear polypropylene that is not folded Then fold the bag in thirds from side to side 2 Set your Heat Sealer to 6 setting may vary depending on heat sealer and power source and press the Heat Sealer arm down for 3 to 4 seconds to seal the unfolded polypropylene to the folded part of the bag 3 Label the folded sealed filter bag and place it in a labeled ashing...

Страница 63: ...ning you must install IDF or IDF Flow Thru bags at all six IDF stations When a digestion process completes the following screen is displayed showing that the process in the top bags is complete The Method Procedure shown in the top right corner will be the one that is currently in progress in the bottom bags At this time you can remove the IDF or IDF Flow Thru bags from the instrument then press t...

Страница 64: ...rm your set up the following screen is displayed The Method Procedure shown in the top right corner will be the one that you just started in the top bags 2 Perform the digestion process using the ANKOMTDF Dietary Fiber Analyzer To perform the new digestion process press the START button to begin The following screen will be displayed After the pump tube testing is complete the Status screen will b...

Страница 65: ...up the following Status screen is displayed The Method Procedure shown in the top right corner is the one that is running in the top bags The instrument is now ready to run an SDF process that will be automatically initiated by the internal computer at the correct time Vacuum sensor AAAA psi Status Overall Progress Action in progress IDF Time remaining Process in progress XX XX XX Time remaining X...

Страница 66: ...ause problems The IDF SDF and TDF analyses both start filling the SDF bag with EtOH about an hour after the IDF process starts Therefore if you start the IDF process and wait more than an hour to properly install your SDF bags the instrument will deliver EtOH to the empty bottom stations wasting EtOH and ruining a run To enter Expert Mode follow the steps below 1 From the Select a Function screen ...

Страница 67: ...isplayed in this box 2 Vacuum sensor pressure The value shown in box 2 is the current pressure in psi units read by the vacuum sensor If an error occurs during operation and the user overrides the error to continue the operation this box will not display a value 3 Function and Procedure Box 3 displays the method name along with the most recent procedure that you have started either IDF SDF or TDF ...

Страница 68: ...l stop the next process from starting While paused the button will read CONT To continue the operation press the CONT button While paused the instrument will continue the mechanical operation of the current step For example if you pause the instrument while mixing is occurring in the IDF process the mixing paddles will continue to function even though the process timer has paused 12 Bottom process...

Страница 69: ...played when a top or bottom process begins its filter action At that time the button reads Filter Time When button 16 is pressed the remaining filter time is increased by one minute for every time the button is pressed up to a limit of 54 additional minutes 17 Filter bypass or Precipitation bypass Button 17 is displayed when a top or bottom process begins its filter action or when a bottom process...

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Страница 71: ...een will NOT show these numbers 1 Fault description Box 1 contains the description of a fault that has recently occurred 2 Reset fault after it has been repaired Button 2 is only displayed when a low input pressure fault occurs When button 2 is pressed the fault is reset 3 Next fault Multiple faults can occur during a procedure Pressing button 3 will walk through all of the existing faults List of...

Страница 72: ...cal FAQs section at www ankom com E10 High Line Pressure The pressure in one or more of the delivery tubes is higher than the specified values In progress processes are aborted See Technical FAQs section at www ankom com E11 Low N2 Input Pressure The pressure at the input port is lower than the specified range In progress processes are frozen in time The mixer is stopped The N2 filter is disabled ...

Страница 73: ...he Control Panel buttons located below the Touch Screen Display are disabled Pressing the buttons on this screen will actuate specific valves with the exception of the Mixer Duty Cycle button Valve Test Amylase Supply open HCl Supply closed AMG Supply open Protease Supply open Clamp Bar A out Clamp Bar D out Clamp Bar C out Clamp Bar B out Water Supply closed EtOH95 Supply closed EtOH78 Supply clo...

Страница 74: ...heater button After completing the Heater Test you should turn all heaters off Faults When you press the Faults button on the Diagnostics screen the Faults screen is displayed See the Fault Handling section of this manual for more information Pressures When you press the Pressures button on the Diagnostics screen the following screen will be displayed The readings shown on this screen for the inpu...

Страница 75: ...his diagnostic also displays the maximum pressure read from the vacuum sensor during the test The value starts at zero and will display a value while the motor is running and when it stops This can be used for troubleshooting Running the Motor Test could leave fluid in some of the lines After running a Motor Test always run the Clear IDF SDF Lines diagnostic Set Valves Amylase Supply closed HCl Su...

Страница 76: ...this Warning Screen is only temporary Use the Digestion Times screen in Diagnostics to make the reset permanent Pump Tube Test When you press the Pump Tube Test button on the Diagnostics screen the following screen will be displayed This diagnostic tests the integrity of the tubing from the pump to the inlet valves IDF SDF and Waste When you press the START button the following screen will be disp...

Страница 77: ...iagnostics screen the following screen will be displayed This diagnostic allows you to pump individual fluids or all of the fluids into the lines from the Chemical Containers to the Tubing Support Panel If by accident you run out of any fluid during a normal IDF SDF or TDF run there may not be enough fluid in the lines for the next assay In that case you should use this diagnostic to re fill the l...

Страница 78: ...en the following screen will be displayed Service Mode Volume Calibration Refer to the QC Calibration Checks section of this manual for details about this diagnostic Service Mode Temperature Sensor Corrections Refer to the QC Calibration Checks section of this manual for details about this diagnostic Service Mode Bypass Amylase Phase Initial Heat up For digestions to occur at the correct temperatu...

Страница 79: ... with a registration number that you will use to configure your instrument to run the AOAC 2009 01 and 2011 25 methods When you press the AOAC 2009 01 2011 25 Registration button on the Service Mode screen the following screen will be displayed To enable the AOAC 2009 01 2011 25 methods enter the 5 digit registration number provided by ANKOM Technology in the white box with the gray border and pre...

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Страница 81: ...observed Inspect tubing for wear or leaks On a monthly basis or more frequently depending on usage follow the steps below to inspect the pneumatic and silicone tubing 1 With the instrument connected to pressurized nitrogen inspect the pressurized air lines Listen for any audible leaks and check for cracks or kinks 2 Run a Pump Tube Test as described in the Diagnostics Mode section of this manual C...

Страница 82: ...The ANKOM Technology web site has the most current troubleshooting and replacement parts information Therefore if you have any questions about the operation of your ANKOMTDF Dietary Fiber Analyzer or if you need replacement parts please visit our web site at www ankom com ...

Страница 83: ...erify that the instrument is delivering the correct amount of fluid through the pump This calibration should be done after installing new pump tubing Refer to the Periodic Maintenance section of this manual for further instructions To run the Volume Calibration procedure follow the steps below 1 Press the Diagnostics button on the screen above The following screen will be displayed 2 Press the Ser...

Страница 84: ...the Volume Calibration screen above The following screen will be displayed Place spare containers under IDF Delivery Nozzles to catch pre calibration charge Press OK when done OK Volume Calibration Volume Calibration xx x Calibration Time START Service Mode Temperature Sensor Corrections IDF Count Volume Calibration Bypass Amylase Phase Initial Heat up NO AOAC 2009 01 2011 25 Registration ...

Страница 85: ...on After fluid empties into the containers the following screen will be displayed 7 Remove the containers with fluid 8 Install the empty pre weighed beakers under all six IDF Delivery Nozzles and press the OK button While the fluids are being delivered the following screen will be displayed Place Calibration containers under the IDF Delivery Nozzles Press OK when done OK Volume Calibration ...

Страница 86: ...ases Because the sensors have a tolerance range you can set offsets to correct for differences in temperature sensors To calibrate the Temperature Sensing system follow the steps below 1 Start an IDF SDF analysis on the instrument without using any samples See the IDF SDF Analysis section of this manual for more details 2 After 15 minutes of time has elapsed in the Protease phase of the IDF proces...

Страница 87: ...oller software version vX XX dd mm yy Pump Tube Test Expert Mode OFF Faults Service Mode Clear IDF SDF lines Digestion Times Heater Test Temperatures Pressures Line Charge Motor Test Valve Test Select a Function AOAC 991 43 IDF SDF AOAC 985 29 TDF Diagnostics AOAC 991 43 TDF AOAC 2001 03 TDF Status Overall Progress Action in progress IDF Time remaining IDF Aborted XX XX XX SDF Time remaining XX XX...

Страница 88: ...th the buffer and enzyme solutions during the IDF process To check the mixing operation of the heating paddles follow the steps below 1 Start an IDF SDF analysis on the instrument without using any samples See the IDF SDF Analysis section of this manual for more details When Clamp Bar A closes at the beginning of the Amylase phase the heating paddles will start actuating 2 Count the number of heat...

Страница 89: ...AOAC 991 43 985 29 and 2001 03 methods With no specific enzyme activity standards referred to in the dietary fiber methods ANKOM has chosen to use the activity numbers specified by Megazyme based on their extensive research As always refer to the specific method for Enzyme purity information and run specified test samples as appropriate a Enzyme solutions To make your enzyme solutions for use in t...

Страница 90: ...Adjust pH to 8 2 with 6N NaOH and dilute to 2 L with H2O Note It is important to adjust pH to 8 2 at 24 C However if buffer temperature is 20 C adjust pH to 8 3 if temperature is 28 C adjust pH to 8 1 For deviations between 20 and 28 C adjust by interpolation i Hydrochloric acid solution AOAC 991 43 0 561N Dilute 93 5 ml of 6N HCl to 1 L with H2O General solutions unique to the AOAC 985 29 and 200...

Страница 91: ...e and AMG buttons on the Touch Screen Display 1 6 Disconnect Enzyme HCl EtOH78 EtOH95 Water and Buffer containers Store or dispose of all fluids properly 1 7 Obtain the TDF70 Line Flush Tubing Assembly shown below Note the nine connection points on the Tubing Assembly 1 8 Attach the Line Flush Tubing Assembly to the instrument and to the 78 EtOH Container as shown above 1 9 Fill the 78 EtOH Contai...

Страница 92: ... are open 2 3 Reinstall the clear back panel and tighten the acorn nuts When returning the instrument to service after a period of non use you must close and latch the pump doors and charge all of the lines before running See the IDF SDF Analysis TDF Analysis or Diagnostics sections of this manual for information about charging the lines Acorn Nuts the top two are behind the black torsion bar Cabl...

Страница 93: ...testing 2 Record the weight of each XT4 Filter Bag It is important to note that the weight of the XT4 Filter Bag must be included in the analysis calculations in the following ways For an IDF SDF analysis add the weight of the XT4 bag to the weight of the IDF bag For a TDF analysis add the weight of the XT4 bag to the weight of the SDF bag 3 Record the weight of each sample to be de fatted in the ...

Страница 94: ...appropriate TDF bag and cut the XT4 into approximately a dozen pieces being careful not to lose any sample If any sample sticks to the scissors brush it off into the TDF bag 8 For a TDF analysis make sure all XT4 pieces drop from the IDF Flow Thru Bags into the SDF Filter Bags Use tongs if necessary to help move the pieces 9 When performing the Ashing procedure use an XT4 ash BLANK value in the ca...

Страница 95: ...DF Filter Bags 50 DF FT Flow thru Filter Bags 100 TDF81 α Amylase 100ml TDF83 Protease 100ml TDF84 Amyloglucosidase 100ml TDF80 α Amylase 20ml TDF82 Protease 20ml TDF84 Amyloglucosidase 20ml TDF86 α Amylase Porcine Pancreatic 2g TDF72 AOAC 2009 01 2011 25 Kit with Registration Code 1 Amylase AMG Container TDF57 1 Trizma Base Container TDF58 TDF97 Glass Filtrate Cup 1 Acetic Acid Container TDF59 Fl...

Страница 96: ...mples at one time RF Gas Production System High sensitivity pressure measurement Applications include Biomass to Energy analysis e g Ethanol methane etc Biodegradability Ruminant Nutrition Yeast Activity Beer Wine Fermentation Soil respiration BOD Human Digestion etc Wireless Computer control and data storage Chemicals A wide variety of chemicals used for many different lab operations Pre mixed so...

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