Agilent Technologies PL-GPC 220, Руководство пользователя

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PL-GPC 220 User Manual 53
Using the Module
4
Running Samples on the PL-GPC 220
Running Samples on the PL-GPC 220
Setting up the Autosampler Sequence
1
Click on the autosampler image on the control software.
2
Set the first and last vials in the autosampler sequence.
You will see the image of the carousel update with yellow sample vials.
3
Select any vial positions that you wish to be rinse vials.
On the carousel image you will see the rinse vials turn blue.
4 Set an equilibration time as required.
5
Set the syringe speed. Typically, this is set at the second highest setting.
6 Set the hot and warm zone temperatures.
7
If you wish the instrument to go into IDLE mode after the autosampler
sequence has finished, tick the
Idle after sequence box (remember to check
the Idle flow rates and Idle oven temperature settings).
8
Set the Run time, typically, the same as the data collection time.
9
Click the
Send
button to update the instrument with the new autosampler
settings.
10 Adjust the autosampler over temperature cut out to 30 °C above the hot
zone temperature.
11 Wait 15 min to ensure that the autosampler has reached the required
operating temperatures.
Preparations • Start the log file to monitor the condition of the instrument during operation.
• Ensure that there is sufficient eluent to carry out the analysis (you can use the time estimator
tool on the Control software once you have defined the autosampler sequence).
• Ensure that there is sufficient capacity in the waste container.
N O T E
Rinse vials contain only solvent and so can be used to flush the injection system.
N O T E
If you are using a viscometer, remember to add time to the Run time to ensure that the
negative DP peak arising from the flushing of the delay volume in the viscometer bridge
elutes before data collection of the next sample in the sequence commences .